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Guide to yeast genetics and molecular biology
edited by Christine Guthrie , Gerald R. Fink
- Publikováno
- San Diego : Academic Press, 1991
- Edice
- Methods in enzymology ; Vol. 194
- Stránkování
- 933 s. : obr., tab.
Jazyk angličtina Země Spojené státy americké
Knihovny.cz ISBN
0-12-182095-5
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study \"Your Favorite Gene\" in yeast. Key Features* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* High-efficiency transformation* Preparation of yeast artificial chromosome vectors* Basic Methods of Cell Biology* Immunomicroscopy* Protein targeting assays* Biochemistry of Gene Expression* Vectors for regulated expression* Isolation of labeled and unlabeled DNA, RNA, and protein
Skrytá bibl.
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