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Laser capture microdissection : methods and protocols
Edited by Graeme I. Murray and Stephanie Curran
- Publikováno
- Totowa : Humana Press, 2005
- Vydání
- [1st ed.]
- Edice
- Methods in molecular biology ; Vol. 293
- Stránkování
- xiv, 319 s. : il.
Jazyk angličtina Země Spojené státy americké
Knihovny.cz ISBN
1-58829-260-6
Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.
Bibliografie, rejstřík
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