• MeSH
• biotechnologie metody   MeSH
• buněčné kultury veterinární   MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Biotechnologie. Genetické inženýrství
• NLK Obory
• biomedicínské inženýrství

Enabling technologies -- Setting up a new cell culture laboratory -- Adaptation of mammalian cells to growth in serum-free media -- Viral evaluation of animal cell lines used in biotechnology -- Molecular methods -- Optimizing gene expression in mammalian cells -- Cytogenetic characterization of recombinant cells -- Immortalization strategies for mammalian cells -- Mouse monoclonal antibodies: immunization and production protocols -- Cell bank preparation and characterization -- DNA fingerprinting and characterization of animals cells -- Cell evaluation protocols -- Cell counting and viability measurements -- Monitoring animal cell growth and productivity by flow cytometry -- Measurement of cell death in culture -- Nuclear magnetic resonance methods -- Specialist techniques -- Culturing animal cells in fluidized bed reactors -- GPI-anchored fusion proteins -- Harvesting GPI-anchored proteins from CHO cells -- Hematopoietic cells for cellular and gene therapy: 1. Basic assay techniques -- Hematopoietic cells for cellular and gene therapy: 2. Expansion protocols -- Cytotoxicity testing using cell lines -- Product evaluation protocols --Measuring the folding dynamics of recombinant proteins secreted from mammalian cells -- Control of proteolysis in cell culture: use of inhibitors and engineered cell lines -- Monitoring recombinant glycoprotein heterogeneity -- Measurement of cell-culture glycosidase activity.