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A new type of migrating zone boundary in electrophoresis: 3. The hybrid boundary and stacking criteria

Gebauer P., Malá Z., Boček P.

. 2006 ; 27 (5-6) : 962-967.

Jazyk angličtina Země Německo

Perzistentní odkaz   https://www.medvik.cz/link/bmc07521396

This article investigates the principles of stacking of analytes by a zone of a bulk sample component that possesses one hybrid boundary. The model system investigated comprises a transient stacking zone in a BGE where the rear boundary of this zone is a hybrid one. A theoretical description of such a system is given, and general rules and mathematical criteria for quantitative stacking are presented. These criteria are based on comparison of migration velocities of analytes to be stacked and velocities of the boundaries of the stacking zone. It is shown that the presence of a hybrid boundary brings about additional constraints for stacking of analytes due to the presence of the sharp part of the hybrid boundary that migrates faster than regular sharp boundary of this zone. An experimental example related to the described theory is also shown.

Citace poskytuje Crossref.org

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