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Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains
A Nemec, M Maixnerova, der Reijden TJ van, den Broek PJ van, L Dijkshoorn
Jazyk angličtina Země Velká Británie
Grantová podpora
NR8554
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
NLK
Free Medical Journals
od 1996 do Před 1 rokem
Open Access Digital Library
od 1996-01-01
- MeSH
- ABC transportéry genetika metabolismus MeSH
- Acinetobacter baumannii genetika metabolismus účinky léků MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny genetika MeSH
- DNA fingerprinting MeSH
- genotyp MeSH
- infekce bakteriemi rodu Acinetobacter mikrobiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence genetika MeSH
- netilmicin farmakologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- upregulace účinky léků MeSH
- Check Tag
- lidé MeSH
OBJECTIVE: To assess the occurrence of the genes of the AdeABC efflux system and their association with antimicrobial resistance in Acinetobacter baumannii. METHODS: A set of 116 strains selected for their diversity both in genotypic properties and geographic origin was investigated for the presence of the structural (adeA, adeB and adeC) and regulatory (adeR and adeS) genes of the AdeABC system by PCR, for resistance to 11 antimicrobials by disc diffusion, for MIC of netilmicin and for the presence of aacC2 and aacA4, encoding netilmicin-modifying enzymes. RESULTS: Ninety-five strains were positive for adeA, adeB, adeR and adeS, 10 were positive for 1 to 3 of these genes and 11 were negative for all genes. The adeC gene was found in 49 strains with one or more of the other genes. Forty-one strains were resistant to a maximum of one agent and 75 strains to two or more agents. Netilmicin MICs showed an almost bimodal distribution with respective peaks of 0.5-1 and 8 mg/L; aacC2 or aacA4 was found in six strains with netilmicin MIC of >or=64 mg/L. All 61 strains with netilmicin MICs >or= 4 mg/L were both adeABRS-positive and resistant to two or more agents, whereas netilmicin MICs
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- $a Nemec, Alexandr $7 xx0079755
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- $a Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains / $c A Nemec, M Maixnerova, der Reijden TJ van, den Broek PJ van, L Dijkshoorn
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- $a Centre of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic. anemec@szu.cz
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- $a OBJECTIVE: To assess the occurrence of the genes of the AdeABC efflux system and their association with antimicrobial resistance in Acinetobacter baumannii. METHODS: A set of 116 strains selected for their diversity both in genotypic properties and geographic origin was investigated for the presence of the structural (adeA, adeB and adeC) and regulatory (adeR and adeS) genes of the AdeABC system by PCR, for resistance to 11 antimicrobials by disc diffusion, for MIC of netilmicin and for the presence of aacC2 and aacA4, encoding netilmicin-modifying enzymes. RESULTS: Ninety-five strains were positive for adeA, adeB, adeR and adeS, 10 were positive for 1 to 3 of these genes and 11 were negative for all genes. The adeC gene was found in 49 strains with one or more of the other genes. Forty-one strains were resistant to a maximum of one agent and 75 strains to two or more agents. Netilmicin MICs showed an almost bimodal distribution with respective peaks of 0.5-1 and 8 mg/L; aacC2 or aacA4 was found in six strains with netilmicin MIC of >or=64 mg/L. All 61 strains with netilmicin MICs >or= 4 mg/L were both adeABRS-positive and resistant to two or more agents, whereas netilmicin MICs <or= 2 mg/L (n = 51) were found for all strains resistant to a maximum of one agent and those negative for one or more of the adeABRS genes. CONCLUSIONS: The AdeABC genes are common in A. baumannii, but may be absent in some, mostly fully susceptible strains. Decreased susceptibility to netilmicin (MIC 4-32 mg/L) is associated with both the presence of these genes and multidrug resistance and may be indicative of AdeABC overexpression.
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- $a infekce bakteriemi rodu Acinetobacter $x mikrobiologie $7 D000151
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- $a Acinetobacter baumannii $x genetika $x metabolismus $x účinky léků $7 D040981
- 650 _2
- $a antibakteriální látky $x farmakologie $7 D000900
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- $a DNA fingerprinting $7 D016172
- 650 _2
- $a mnohočetná bakteriální léková rezistence $x genetika $7 D024901
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- $a bakteriální geny $x genetika $7 D005798
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- $a genotyp $7 D005838
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- $a lidé $7 D006801
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- $a mikrobiální testy citlivosti $7 D008826
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- $a netilmicin $x farmakologie $7 D009428
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- $a polymerázová řetězová reakce s reverzní transkripcí $7 D020133
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- $a upregulace $x účinky léků $7 D015854
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- $a Maixnerová, Martina $7 xx0093284
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- $a Reijden, Tanny J. K. van der
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- $a Broek, Peterhans J. van den
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- $a Dijkshoorn, Lenie
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