-
Je něco špatně v tomto záznamu ?
Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells
E Valouskova, K Smolkova, J Santorova, P Jezek, M Modriansky
Jazyk angličtina Země Slovensko
- MeSH
- apoptóza MeSH
- buněčné jádro metabolismus MeSH
- financování organizované MeSH
- HeLa buňky MeSH
- lidé MeSH
- mitochondrie metabolismus MeSH
- proteiny regulující apoptózu biosyntéza metabolismus MeSH
- transport proteinů MeSH
- Check Tag
- lidé MeSH
Cell death-inducing DFF[DNA fragmentation factor]-like effector-a (CIDEa), may initiate apoptosis by disrupting a complex consisting of 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD). CIDEa, however, was found to be localized in mitochondria. We have performed immunodetection of CIDEa in whole cells and subcellular fractions of HeLa cells adapted for a tetracycline-inducible CIDEa expression. Using immunocytochemistry we observed redistribution, enhanced upon treatment with camptothecin or valinomycin, of CIDEa to nucleus. Similarly, CIDEa content increased in the nuclear fraction but decreased in cytosolic fraction in cells treated to initiate apoptosis. We hypothesize that CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis.
- 000
- 02382naa 2200349 a 4500
- 001
- bmc11005090
- 003
- CZ-PrNML
- 005
- 20111210202820.0
- 008
- 110311s2008 xo e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xo
- 100 1_
- $a Gabrielová, Eva. $7 xx0267682
- 245 10
- $a Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells / $c E Valouskova, K Smolkova, J Santorova, P Jezek, M Modriansky
- 314 __
- $a Institute of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. walouch@email.cz
- 520 9_
- $a Cell death-inducing DFF[DNA fragmentation factor]-like effector-a (CIDEa), may initiate apoptosis by disrupting a complex consisting of 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD). CIDEa, however, was found to be localized in mitochondria. We have performed immunodetection of CIDEa in whole cells and subcellular fractions of HeLa cells adapted for a tetracycline-inducible CIDEa expression. Using immunocytochemistry we observed redistribution, enhanced upon treatment with camptothecin or valinomycin, of CIDEa to nucleus. Similarly, CIDEa content increased in the nuclear fraction but decreased in cytosolic fraction in cells treated to initiate apoptosis. We hypothesize that CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis.
- 650 _2
- $a apoptóza $7 D017209
- 650 _2
- $a proteiny regulující apoptózu $x biosyntéza $x metabolismus $7 D051017
- 650 _2
- $a buněčné jádro $x metabolismus $7 D002467
- 650 _2
- $a HeLa buňky $7 D006367
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mitochondrie $x metabolismus $7 D008928
- 650 _2
- $a transport proteinů $7 D021381
- 650 _2
- $a financování organizované $7 D005381
- 700 1_
- $a Smolková, Katarína $7 xx0139595
- 700 1_
- $a Šantorová, Jitka $7 xx0127127
- 700 1_
- $a Ježek, Petr, $d 1945- $7 xx0030581
- 700 1_
- $a Modrianský, Martin, $d 1966- $7 xx0042117
- 773 0_
- $t General Physiology & Biophysics $w MED00001896 $g Roč. 27 (2008), s. 92-100 $x 0231-5882
- 910 __
- $a ABA008 $b B 1566 $y 1
- 990 __
- $a 20110414094407 $b ABA008
- 991 __
- $a 20110414094407 $b ABA008
- 999 __
- $a ok $b bmc $g 832940 $s 697140
- BAS __
- $a 3
- BMC __
- $a 2008 $b 27 $d 92-100 $i 0231-5882 $m General physiology and biophysics $n Gen Physiol Biophys $x MED00001896
- LZP __
- $a 2011-4B/vtme
