• Something wrong with this record ?

Comparative proteomic profiling of culture filtrate proteins of less and highly virulent Francisella tularensis strains

K. Konecna, L. Hernychova, M. Reichelova, J. Lenco, J. Klimentova, J. Stulik, A. Macela, T. Alefantis, VG. Delvecchio

. 2010 ; 10 (24) : 4501-4511. [pub] 20101109

Language English Country Germany

Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12026600

The facultative intracellular bacterium Francisella tularensis is the causal agent of the serious infectious disease tularemia. Despite the dynamic progress, which has been made in last few years, important questions regarding Francisella pathogenicity still remain to be answered. Generally, secreted proteins play an important role in pathogenicity of intracellular microbes. In this study, we investigated the protein composition of the culture filtrate proteins of highly virulent F. tularensis subsp. tularensis, strain SCHU S4 and attenuated F. tularensis subsp. holarctica, live vaccine strain using a comparative proteomic analysis. The majority of proteins identified in this study have been implicated in virulence mechanisms of other pathogens, and several have been categorized as having moonlighting properties; those that have more than one unrelated function. This profiling study of secreted proteins resulted in the unique detection of acid phosphatase (precursor) A (AcpA), β-lactamase, and hypothetical protein FTT0484 in the highly virulent strain SCHU S4 secretome. The release of AcpA may be of importance for F. tularensis subsp. tularensis virulence due to the recently described AcpA role in the F. tularensis escape from phagosomes.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12026600
003      
CZ-PrNML
005      
20160301055921.0
007      
ta
008      
120816s2010 gw f 000 0#eng||
009      
AR
024    7_
$a 10.1002/pmic.201000248 $2 doi
035    __
$a (PubMed)21136602
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Konecna, Klara $u Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
245    10
$a Comparative proteomic profiling of culture filtrate proteins of less and highly virulent Francisella tularensis strains / $c K. Konecna, L. Hernychova, M. Reichelova, J. Lenco, J. Klimentova, J. Stulik, A. Macela, T. Alefantis, VG. Delvecchio
520    9_
$a The facultative intracellular bacterium Francisella tularensis is the causal agent of the serious infectious disease tularemia. Despite the dynamic progress, which has been made in last few years, important questions regarding Francisella pathogenicity still remain to be answered. Generally, secreted proteins play an important role in pathogenicity of intracellular microbes. In this study, we investigated the protein composition of the culture filtrate proteins of highly virulent F. tularensis subsp. tularensis, strain SCHU S4 and attenuated F. tularensis subsp. holarctica, live vaccine strain using a comparative proteomic analysis. The majority of proteins identified in this study have been implicated in virulence mechanisms of other pathogens, and several have been categorized as having moonlighting properties; those that have more than one unrelated function. This profiling study of secreted proteins resulted in the unique detection of acid phosphatase (precursor) A (AcpA), β-lactamase, and hypothetical protein FTT0484 in the highly virulent strain SCHU S4 secretome. The release of AcpA may be of importance for F. tularensis subsp. tularensis virulence due to the recently described AcpA role in the F. tularensis escape from phagosomes.
650    _2
$a bakteriální proteiny $x chemie $7 D001426
650    _2
$a kultivační média speciální $7 D017077
650    _2
$a Francisella tularensis $x chemie $x patogenita $7 D005604
650    _2
$a proteom $x chemie $7 D020543
650    _2
$a faktory virulence $x chemie $7 D037521
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hernychová, Lenka, $d 1965- $7 xx0073983 $u Institute for Radiobiology and Immunology, Purkynĕ Military Medical Academy, Trebesská 1575, 500 01 Hradec Králové, Czech Republic
700    1_
$a Reichelová, Markéta $7 _BN002352
700    1_
$a Lenčo, Juraj, $d 1979- $7 mzk2007377664 $u Faculty of Military Health Sciences, Institute of Molecular Pathology, University of Defense, Hradec Kralove, Czech Republic
700    1_
$a Klimentová, Jana $7 xx0135751 $u Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: jana.klimentova@unob.cz
700    1_
$a Stulík, Jiří, $d 1957- $7 mzk2008473161 $u Faculty of Military Health Sciences, Institute of Molecular Pathology, University of Defense, Hradec Kralove, Czech Republic
700    1_
$a Macela, Aleš, $d 1944- $7 mzk2005279505 $u Institute for Immunology, Purkyne Military Medical Academy, Czech Republic
700    1_
$a Alefantis, Tim $u Vital Probes, Inc., 1300 Old Plank Road, Mayfield, PA 18433, USA $7 gn_A_00003701
700    1_
$a Delvecchio, Vito G. $u Vital Probes, Mayfield, Pennsylvania, USA
773    0_
$w MED00007044 $t Proteomics $x 1615-9861 $g Roč. 10, č. 24 (2010), s. 4501-4511
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21136602 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160301055934 $b ABA008
999    __
$a ok $b bmc $g 948642 $s 783946
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2010 $b 10 $c 24 $d 4501-4511 $e 20101109 $i 1615-9861 $m Proteomics $n Proteomics $x MED00007044
LZP    __
$b NLK122 $a Pubmed-20120816/11/01

Find record

Citation metrics

Archiving options