• Je něco špatně v tomto záznamu ?

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver

L. Trnkova, S. Krizkova, V. Adam, J. Hubalek, R. Kizek

. 2011 ; 26 (5) : 2201-2207.

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027309

In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals--labelled as cys(MT) and W(a)--were observed. The cys(MT) corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 μg/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12027309
003      
CZ-PrNML
005      
20160318160814.0
007      
ta
008      
120816s2011 enk f 000 0#eng||
009      
AR
024    7_
$a 10.1016/j.bios.2010.09.035 $2 doi
035    __
$a (PubMed)20970982
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Trnková, Libuše. $7 jn20001005289 $u Department of Chemistry, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
245    10
$a Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver / $c L. Trnkova, S. Krizkova, V. Adam, J. Hubalek, R. Kizek
520    9_
$a In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals--labelled as cys(MT) and W(a)--were observed. The cys(MT) corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 μg/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%.
650    _2
$a protilátky $x chemie $x imunologie $7 D000906
650    _2
$a biosenzitivní techniky $x přístrojové vybavení $7 D015374
650    _2
$a uhlík $x chemie $7 D002244
650    _2
$a konduktometrie $x přístrojové vybavení $7 D003217
650    _2
$a elektrody $7 D004566
650    _2
$a design vybavení $7 D004867
650    _2
$a analýza selhání vybavení $7 D019544
650    _2
$a imunoanalýza $x přístrojové vybavení $7 D007118
650    _2
$a metalothionein $x chemie $x imunologie $7 D008668
650    _2
$a vazba proteinů $7 D011485
650    _2
$a stříbro $x analýza $x imunologie $7 D012834
650    _2
$a povrchové vlastnosti $7 D013499
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Křížková, Soňa, $d 1981- $7 xx0105812 $u Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00 Brno, Czech Republic
700    1_
$a Adam, Vojtěch, $d 1982- $7 xx0064599 $u Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00 Brno, Czech Republic
700    1_
$a Hubálek, Jaromír $7 mzk2008486353 $u Department of Microelectronics, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
700    1_
$a Kizek, René, $d 1972- $7 jn20001005291 $u Department of Chemistry, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic; Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00 Brno, Czech Republic
773    0_
$w MED00006627 $t Biosensors & bioelectronics $x 1873-4235 $g Roč. 26, č. 5 (2011), s. 2201-2207
856    41
$u https://pubmed.ncbi.nlm.nih.gov/20970982 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160318160533 $b ABA008
999    __
$a ok $b bmc $g 949351 $s 784655
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 26 $c 5 $d 2201-2207 $i 1873-4235 $m Biosensors & bioelectronics $n Biosens Bioelectron $x MED00006627
LZP    __
$b NLK112 $a Pubmed-20120816/11/02

Najít záznam

Citační ukazatele

Možnosti archivace