Fluorescent Pseudomonas putida CCM 3656 (ATCC 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpoB and rrs genes, manual ribotyping using endonuclease HindIII, DNA base composition determination and DNA-DNA hybridization. The results obtained by these genotyping methods showed that the strain CCM 3656 is distant from P. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profile analysis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry profiling and extended biotyping. The DNA-DNA hybridization experiments performed between the strain CCM 3656 and the closest relatives revealed 77 % similarity with Pseudomonas jessenii. However, the outcomes of sequencing, ribotyping and phenotype characterization allow distinguishing the studied strain from P. jessenii. On the basis of the obtained taxonomic data, we suggest reclassifying strain CCM 3656 to a novel subspecies of P. jessenii and propose naming P. jessenii subsp. pseudoputida subsp. nov. with CCM 3656(T) as type strain. Furthermore, we present an amended description of P. jessenii and proposal of P. jessenii subsp. jessenii subsp. nov.

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