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Formation of indigo by recombinant mammalian cytochrome P450
EM Gillam, AM Aguinaldo, LM Notley, D Kim, RG Mundkowski, AA Volkov, FH Arnold, P Soucek, JJ DeVoss, FP Guengerich
Jazyk angličtina Země Spojené státy americké
Typ dokumentu práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
IZ1850
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
NLK
ScienceDirect (archiv)
od 1993-01-01 do 2009-12-31
PubMed
10558891
Knihovny.cz E-zdroje
- MeSH
- biologické pigmenty * metabolismus MeSH
- cytochrom P-450 CYP2E1 genetika metabolismus MeSH
- Escherichia coli genetika metabolismus MeSH
- geny MeSH
- indigotindisulfonát sodný MeSH
- indoly * metabolismus MeSH
- lidé MeSH
- NADPH-cytochrom c-reduktasa genetika metabolismus MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- systém (enzymů) cytochromů P-450 genetika metabolismus MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
The development of bicistronic systems for coexpression of recombinant human cytochrome P450 enzymes (P450s) with their redox partner, NADPH-cytochrome P450 reductase (NPR), has enabled P450 activity to be reconstituted within bacterial cells. During expression of recombinant P450 2E1 and some other forms, we observed the formation of a blue pigment in bacterial cultures. The pigment was extracted from cultures and shown to comigrate with standard indigo on TLC. UV-visible spectroscopy and mass spectrometric analysis provided further support for identification of the pigment as indigo. Indigo is known to form following the spontaneous oxidation of 3-hydroxyindole. Accordingly, we speculated that indole, formed as a breakdown product of tryptophan in bacteria, was hydroxylated by the P450 system, leading to indigo formation. Bacterial membranes containing recombinant P450 2E1 and human NPR were incubated in vitro with indole and shown to catalyze formation of a blue pigment in a time- and cofactor-dependent manner. These studies suggest potential applications of mammalian P450 enzymes in industrial indigo production or in the development of novel colorimetric assays based on indole hydroxylation. Copyright 1999 Academic Press.
Department of Chemistry University of Queensland Brisbane Queensland Australia
National Institute of Public Health Center of Occupational Diseases Praha Czech Republic
Literatura
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