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Pseudomonas prosekii sp. nov., a novel psychrotrophic bacterium from Antarctica
M. Kosina, M. Barták, I. Mašlaňová, AV. Pascutti, O. Sedo, M. Lexa, I. Sedláček,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
- MeSH
- DNA bakterií chemie genetika MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- fylogeneze MeSH
- hybridizace nukleových kyselin MeSH
- mikrobiologie životního prostředí * MeSH
- molekulární sekvence - údaje MeSH
- Pseudomonas klasifikace genetika izolace a purifikace fyziologie MeSH
- ribotypizace MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- sigma faktor genetika MeSH
- techniky typizace bakterií MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Antarktida MeSH
During Czech expeditions at James Ross Island, Antarctica, in the years 2007-2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA-DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37 °C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423(T) showed only 40.9-50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1(T) (=CCM 7990(T) and LMG 26867(T)).
Citace poskytuje Crossref.org
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