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Structure-function relationships during transgenic telomerase expression in Arabidopsis
D. Zachová, M. Fojtová, M. Dvořáčková, I. Mozgová, I. Lermontova, V. Peška, I. Schubert, J. Fajkus, E. Sýkorová,
Language English Country Denmark
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23278240
DOI
10.1111/ppl.12021
Knihovny.cz E-resources
- MeSH
- Arabidopsis enzymology genetics MeSH
- Cell Nucleolus enzymology genetics MeSH
- Cell Nucleus enzymology genetics MeSH
- Plants, Genetically Modified MeSH
- Nuclear Localization Signals genetics MeSH
- Catalytic Domain genetics MeSH
- Plant Leaves genetics MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Protein Biosynthesis MeSH
- Gene Expression Regulation, Plant MeSH
- RNA Splicing MeSH
- Nicotiana genetics MeSH
- Telomerase chemistry genetics metabolism MeSH
- Protein Structure, Tertiary MeSH
- Structure-Activity Relationship MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Although telomerase (EC 2.7.7.49) is important for genome stability and totipotency of plant cells, the principles of its regulation are not well understood. Therefore, we studied subcellular localization and function of the full-length and truncated variants of the catalytic subunit of Arabidopsis thaliana telomerase, AtTERT, in planta. Our results show that multiple sites in AtTERT may serve as nuclear localization signals, as all the studied individual domains of the AtTERT were targeted to the nucleus and/or the nucleolus. Although the introduced genomic or cDNA AtTERT transgenes display expression at transcript and protein levels, they are not able to fully complement the lack of telomerase functions in tert -/- mutants. The failure to reconstitute telomerase function in planta suggests a more complex telomerase regulation in plant cells than would be expected based on results of similar experiments in mammalian model systems.
References provided by Crossref.org
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