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Der p 11 is a major allergen for house dust mite-allergic patients suffering from atopic dermatitis
S. Banerjee, Y. Resch, KW. Chen, I. Swoboda, M. Focke-Tejkl, K. Blatt, N. Novak, M. Wickman, M. van Hage, R. Ferrara, A. Mari, A. Purohit, G. Pauli, EN. Sibanda, P. Ndlovu, WR. Thomas, V. Krzyzanek, S. Tacke, U. Malkus, P. Valent, R. Valenta, S. Vrtala,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1938 to 1 year ago
Freely Accessible Science Journals
from 1938 to 1 year ago
ProQuest Central
from 2000-01-01 to 2015-12-31
Open Access Digital Library
from 1938-01-01
Health & Medicine (ProQuest)
from 2000-01-01 to 2015-12-31
PubMed
24999597
DOI
10.1038/jid.2014.271
Knihovny.cz E-resources
- MeSH
- Antigens, Dermatophagoides chemistry genetics immunology MeSH
- Dermatitis, Atopic epidemiology immunology MeSH
- Biomarkers MeSH
- Circular Dichroism MeSH
- Dermatophagoides pteronyssinus genetics immunology MeSH
- Child MeSH
- Adult MeSH
- Immunoglobulin E blood immunology MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Molecular Sequence Data MeSH
- Arthropod Proteins MeSH
- Antibodies blood immunology MeSH
- Protein Structure, Secondary MeSH
- Amino Acid Sequence MeSH
- Seroepidemiologic Studies MeSH
- Tropomyosin chemistry genetics immunology MeSH
- Animals MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
House dust mites (HDMs) belong to the most potent indoor allergen sources worldwide and are associated with allergic manifestations in the respiratory tract and the skin. Here we studied the importance of the high-molecular-weight group 11 allergen from Dermatophagoides pteronyssinus (Der p 11) in HDM allergy. Sequence analysis showed that Der p 11 has high homology to paramyosins from mites, ticks, and other invertebrates. A synthetic gene coding for Der p 11 was expressed in Escherichia coli and rDer p 11 purified to homogeneity as folded, alpha-helical protein as determined by circular dichroism spectroscopy. Using antibodies raised against rDer p 11 and immunogold electron microscopy, the allergen was localized in the muscle beneath the skin of mite bodies but not in feces. IgE reactivity of rDer p 11 was tested with sera from HDM-allergic patients from Europe and Africa in radioallergosorbent test-based dot-blot assays. Interestingly, we found that Der p 11 is a major allergen for patients suffering from atopic dermatitis (AD), whereas it is only a minor allergen for patients suffering from respiratory forms of HDM allergy. Thus, rDer p 11 might be a useful serological marker allergen for the identification of a subgroup of HDM-allergic patients suffering from HDM-associated AD.
Center for Molecular Allergology IDI IRCCS Rome Italy
Christian Doppler Laboratory for Allergy Research Medical University of Vienna Vienna Austria
Department of Dermatology and Allergy University of Bonn Bonn Germany
Gamma City Laboratory Harare Zimbabwe
Institute of Medical Physics and Biophysics University of Münster Münster Germany
Institute of Scientific Instruments Academy of Sciences of the Czech Republic Brno Czech Republic
Service de Pneumologie Hôpitaux Universitaires de Strasbourg Strasbourg France
References provided by Crossref.org
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