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Novel functions of an iron-sulfur flavoprotein from Trichomonas vaginalis hydrogenosomes
T. Smutná, K. Pilarová, J. Tarábek, J. Tachezy, I. Hrdý,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1972 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1972 to 6 months ago
Europe PubMed Central
from 1972 to 6 months ago
Open Access Digital Library
from 1972-01-01
Open Access Digital Library
from 1972-01-01
PubMed
24663020
DOI
10.1128/aac.02320-13
Knihovny.cz E-resources
- MeSH
- Antitrichomonal Agents pharmacology MeSH
- Ferredoxins metabolism MeSH
- Flavoproteins metabolism MeSH
- Genes, Fungal MeSH
- Catalysis MeSH
- Drug Resistance MeSH
- Metronidazole chemistry pharmacology MeSH
- Molecular Sequence Data MeSH
- NAD metabolism MeSH
- Iron-Sulfur Proteins metabolism MeSH
- Amino Acid Sequence MeSH
- Subcellular Fractions drug effects metabolism MeSH
- Trichomonas vaginalis metabolism MeSH
- Hydrogen metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Iron-sulfur flavoproteins (Isf) are flavin mononucleotide (FMN)- and FeS cluster-containing proteins commonly encountered in anaerobic prokaryotes. However, with the exception of Isf from Methanosarcina thermophila, which participates in oxidative stress management by removing oxygen and hydrogen peroxide, none of these proteins has been characterized in terms of function. Trichomonas vaginalis, a sexually transmitted eukaryotic parasite of humans, was found to express several iron-sulfur flavoprotein (TvIsf) homologs in its hydrogenosomes. We show here that in addition to having oxygen-reducing activity, the recombinant TvIsf also functions as a detoxifying reductase of metronidazole and chloramphenicol, both of which are antibiotics effective against a variety of anaerobic microbes. TvIsf can utilize both NADH and reduced ferredoxin as electron donors. Given the prevalence of Isf in anaerobic prokaryotes, we propose that these proteins are central to a novel defense mechanism against xenobiotics.
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