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Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro
J. Trylcova, P. Busek, K. Smetana, E. Balaziova, B. Dvorankova, A. Mifkova, A. Sedo,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT12237
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ProQuest Central
from 1997-12-01 to 2015-12-31
Medline Complete (EBSCOhost)
from 2005-01-01 to 2016-12-31
Health & Medicine (ProQuest)
from 1997-12-01 to 2015-12-31
Public Health Database (ProQuest)
from 1997-12-01 to 2015-12-31
ROAD: Directory of Open Access Scholarly Resources
from 1987
- MeSH
- Actins biosynthesis MeSH
- Fibroblasts pathology MeSH
- Glioblastoma genetics pathology MeSH
- Culture Media, Conditioned * MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Tumor Microenvironment genetics MeSH
- Cell Movement * MeSH
- Cell Proliferation genetics MeSH
- Gene Expression Regulation, Neoplastic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cancer-associated fibroblasts (CAFs) significantly influence biological properties of many tumors. The role of these mesenchymal cells is also anticipated in human gliomas. To evaluate the putative role of CAFs in glioblastoma, we tested the effect of CAF conditioned media on the proliferation and chemotaxis of glioma cells. The proliferation of glioma cells was stimulated to similar extent by both the normal fibroblasts (NFs) and CAF-conditioned media. Nevertheless, CAF-conditioned media enhanced the chemotactic migration of glioma cells significantly more potently than the media from normal fibroblasts. In order to determine whether CAF-like cells are present in human glioblastomas, immunofluorescence staining was performed on tissue samples from 20 patients using markers typical for CAFs. This analysis revealed regular presence of mesenchymal cells expressing characteristic CAF markers α-smooth muscle actin and TE-7 in human glioblastomas. These observations indicate the potential role of CAF-like cells in glioblastoma biology.
References provided by Crossref.org
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- $a Cancer-associated fibroblasts (CAFs) significantly influence biological properties of many tumors. The role of these mesenchymal cells is also anticipated in human gliomas. To evaluate the putative role of CAFs in glioblastoma, we tested the effect of CAF conditioned media on the proliferation and chemotaxis of glioma cells. The proliferation of glioma cells was stimulated to similar extent by both the normal fibroblasts (NFs) and CAF-conditioned media. Nevertheless, CAF-conditioned media enhanced the chemotactic migration of glioma cells significantly more potently than the media from normal fibroblasts. In order to determine whether CAF-like cells are present in human glioblastomas, immunofluorescence staining was performed on tissue samples from 20 patients using markers typical for CAFs. This analysis revealed regular presence of mesenchymal cells expressing characteristic CAF markers α-smooth muscle actin and TE-7 in human glioblastomas. These observations indicate the potential role of CAF-like cells in glioblastoma biology.
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