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Influence of a storage protocol on sarcosine levels in the human urinary specimens
Natalia Cernei, Lukas Nejdl, Sona Krizkova, Branislav Ruttkay-Nedecky, Zbynek Heger, Ondrej Zitka, Vojtech Adam
Language English Country Czech Republic
Document type Evaluation Study, Research Support, Non-U.S. Gov't
NLK
Open Access Digital Library
from 2014-01-01
- MeSH
- Time Factors MeSH
- Chromatography, Ion Exchange * statistics & numerical data MeSH
- Electrochemical Techniques statistics & numerical data MeSH
- Humans MeSH
- Metabolomics MeSH
- Biomarkers, Tumor metabolism urine MeSH
- Prostatic Neoplasms metabolism urine MeSH
- Specimen Handling * methods MeSH
- Sarcosine * metabolism urine MeSH
- Temperature MeSH
- Thermodynamics MeSH
- Freezing MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
Urinary metabolomic profiles have recently drawn a lot of attention owing to a debate regarding their possible role as potential clinical markers for prostate cancer. As was shown, amino acid metabolism in cancer patients differs from that in healthy people, and it can be thus utilized in early diagnostics. In this study, we monitored the behavior of potential noninvasive biomarker for prostate carcinoma, sarcosine, involved in the folate metabolism and DNA methylation processes, linked to the progression of prostate carcinoma. To obtain the maximum amount of information, the biochemical parameters (total protein, creatinine, ions, conductivity) were determined using spectrophotometry and electrochemistry. All results were subjected to statistical processing for revealing different correlations between the studied parameters. These metabolites were observed in the urine obtained from healthy subjects and influence of storage conditions (freezing and thawing) on the concentration of addition of sarcosine was monitored.
Central European Institute of Technology Brno University of Technology Czech Republic European Union
Department of Chemistry and Biochemistry Mendel University in Brno Czech Republic European Union
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- $a Cernei, Natalia $7 _AN063287 $u Department of Chemistry and Biochemistry, Mendel University in Brno, Czech Republic - European Union; 2 Central European Institute of Technology, Brno University of Technology, Czech Republic - European Union
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- $a Influence of a storage protocol on sarcosine levels in the human urinary specimens / $c Natalia Cernei, Lukas Nejdl, Sona Krizkova, Branislav Ruttkay-Nedecky, Zbynek Heger, Ondrej Zitka, Vojtech Adam
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- $a Urinary metabolomic profiles have recently drawn a lot of attention owing to a debate regarding their possible role as potential clinical markers for prostate cancer. As was shown, amino acid metabolism in cancer patients differs from that in healthy people, and it can be thus utilized in early diagnostics. In this study, we monitored the behavior of potential noninvasive biomarker for prostate carcinoma, sarcosine, involved in the folate metabolism and DNA methylation processes, linked to the progression of prostate carcinoma. To obtain the maximum amount of information, the biochemical parameters (total protein, creatinine, ions, conductivity) were determined using spectrophotometry and electrochemistry. All results were subjected to statistical processing for revealing different correlations between the studied parameters. These metabolites were observed in the urine obtained from healthy subjects and influence of storage conditions (freezing and thawing) on the concentration of addition of sarcosine was monitored.
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