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Targeted Metabolomics for Homocysteine-Related Metabolites in Primary Hepatocytes
I. Selicharová, M. Kořínek,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromatography, Liquid MeSH
- Hepatocytes metabolism MeSH
- Mass Spectrometry MeSH
- Homocysteine metabolism MeSH
- Humans MeSH
- Metabolome * MeSH
- Metabolomics * methods MeSH
- Primary Cell Culture MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Liquid chromatography-tandem mass spectrometry has become the most convenient method to identify and quantify low molecular weight metabolites from various sources. Metabolomics studies of hepatocytes hold promise for the identification of the mechanisms of toxicant-related disease processes. In this chapter, we present a rapid and sensitive liquid chromatography-tandem mass spectrometry method for the quantification of intracellular concentrations of nine homocysteine-based metabolites, namely homocysteine, methionine, cysteine, dimethylglycine, cystathionine, S-adenosylmethionine, S-adenosylhomocysteine, choline, and betaine. The method is specifically designed for the analysis of cultured primary hepatocytes.
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