-
Je něco špatně v tomto záznamu ?
Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
L. Gerzova, V. Babak, K. Sedlar, M. Faldynova, P. Videnska, D. Cejkova, AN. Jensen, M. Denis, A. Kerouanton, A. Ricci, V. Cibin, J. Österberg, I. Rychlik,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 2006
Public Library of Science (PLoS) od 2006
PubMed Central od 2006
Europe PubMed Central od 2006
ProQuest Central od 2006-12-01
Open Access Digital Library od 2006-01-01
Open Access Digital Library od 2006-10-01
Open Access Digital Library od 2006-01-01
Medline Complete (EBSCOhost) od 2008-01-01
Nursing & Allied Health Database (ProQuest) od 2006-12-01
Health & Medicine (ProQuest) od 2006-12-01
Public Health Database (ProQuest) od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources od 2006
Odkazy
PubMed
26218075
DOI
10.1371/journal.pone.0132892
Knihovny.cz E-zdroje
- MeSH
- bakteriální geny genetika MeSH
- bakteriální léková rezistence genetika MeSH
- biozemědělství * MeSH
- chov zvířat * MeSH
- Evropská unie MeSH
- feces mikrobiologie MeSH
- mikrobiota genetika MeSH
- prasata mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
One of the recent trends in animal production is the revival of interest in organic farming. The increased consumer interest in organic animal farming is mainly due to concerns about animal welfare and the use of antibiotics in conventional farming. On the other hand, providing animals with a more natural lifestyle implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity within fecal microbiota in pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. The abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determined in 468 samples by real-time PCR and the fecal microbiota diversity was characterized in 48 selected samples by pyrosequencing of V3/V4 regions of 16S rRNA. Contrary to our expectations, there were no extensive differences between the abundance of tested antibiotic resistance genes in microbiota originating from organic or conventionally housed pigs within individual countries. There were also no differences in the microbiota composition of organic and conventional pigs. The only significant difference was the difference in the abundance of antibiotic resistance genes in the samples from different countries. Fecal microbiota in the samples originating from southern European countries (Italy, France) exhibited significantly higher antibiotic resistance gene abundance than those from northern parts of Europe (Denmark, Sweden). Therefore, the geographical location of the herd influenced the antibiotic resistance in the fecal microbiota more than farm's status as organic or conventional.
Anses Hygiene and Quality of Poultry and Pig Products Unit Ploufragan France
Brno University of Technology Brno Czech Republic
Istituto Zooprofilattico Sperimentale delle Venezie Padova Italy
National Veterinary Institute Uppsala Sweden
Technical University of Denmark National Food Institute Copenhagen Denmark
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020475
- 003
- CZ-PrNML
- 005
- 20160722120051.0
- 007
- ta
- 008
- 160722s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1371/journal.pone.0132892 $2 doi
- 024 7_
- $a 10.1371/journal.pone.0132892 $2 doi
- 035 __
- $a (PubMed)26218075
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Gerzova, Lenka $u Veterinary Research Institute, Brno, Czech Republic.
- 245 10
- $a Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries / $c L. Gerzova, V. Babak, K. Sedlar, M. Faldynova, P. Videnska, D. Cejkova, AN. Jensen, M. Denis, A. Kerouanton, A. Ricci, V. Cibin, J. Österberg, I. Rychlik,
- 520 9_
- $a One of the recent trends in animal production is the revival of interest in organic farming. The increased consumer interest in organic animal farming is mainly due to concerns about animal welfare and the use of antibiotics in conventional farming. On the other hand, providing animals with a more natural lifestyle implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity within fecal microbiota in pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. The abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determined in 468 samples by real-time PCR and the fecal microbiota diversity was characterized in 48 selected samples by pyrosequencing of V3/V4 regions of 16S rRNA. Contrary to our expectations, there were no extensive differences between the abundance of tested antibiotic resistance genes in microbiota originating from organic or conventionally housed pigs within individual countries. There were also no differences in the microbiota composition of organic and conventional pigs. The only significant difference was the difference in the abundance of antibiotic resistance genes in the samples from different countries. Fecal microbiota in the samples originating from southern European countries (Italy, France) exhibited significantly higher antibiotic resistance gene abundance than those from northern parts of Europe (Denmark, Sweden). Therefore, the geographical location of the herd influenced the antibiotic resistance in the fecal microbiota more than farm's status as organic or conventional.
- 650 12
- $a chov zvířat $7 D000822
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a bakteriální léková rezistence $x genetika $7 D024881
- 650 _2
- $a Evropská unie $7 D005062
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a bakteriální geny $x genetika $7 D005798
- 650 _2
- $a mikrobiota $x genetika $7 D064307
- 650 12
- $a biozemědělství $7 D058871
- 650 _2
- $a RNA ribozomální 16S $x genetika $7 D012336
- 650 _2
- $a prasata $x mikrobiologie $7 D013552
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Babak, Vladimir $u Veterinary Research Institute, Brno, Czech Republic.
- 700 1_
- $a Sedlar, Karel $u Brno University of Technology, Brno, Czech Republic.
- 700 1_
- $a Faldynova, Marcela $u Veterinary Research Institute, Brno, Czech Republic.
- 700 1_
- $a Videnska, Petra $u Veterinary Research Institute, Brno, Czech Republic.
- 700 1_
- $a Cejkova, Darina $u Veterinary Research Institute, Brno, Czech Republic.
- 700 1_
- $a Jensen, Annette Nygaard $u Technical University of Denmark, National Food Institute, Copenhagen, Denmark.
- 700 1_
- $a Denis, Martine $u Anses, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France.
- 700 1_
- $a Kerouanton, Annaelle $u Anses, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France.
- 700 1_
- $a Ricci, Antonia $u Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy.
- 700 1_
- $a Cibin, Veronica $u Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy.
- 700 1_
- $a Österberg, Julia $u National Veterinary Institute (SVA), Uppsala, Sweden.
- 700 1_
- $a Rychlik, Ivan $u Veterinary Research Institute, Brno, Czech Republic.
- 773 0_
- $w MED00180950 $t PloS one $x 1932-6203 $g Roč. 10, č. 7 (2015), s. e0132892
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26218075 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20160722120305 $b ABA008
- 999 __
- $a ok $b bmc $g 1155145 $s 945003
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 10 $c 7 $d e0132892 $e 20150728 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
- LZP __
- $a Pubmed-20160722