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Tracking Mitochondrial DNA In Situ
A. Ligasová, K. Koberna,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Staining and Labeling methods MeSH
- Biotin chemistry MeSH
- Bromodeoxyuridine chemistry MeSH
- DNA Polymerase I metabolism MeSH
- Indoles chemistry MeSH
- Cells, Cultured MeSH
- Oxygen chemistry MeSH
- Humans MeSH
- Copper chemistry MeSH
- DNA, Mitochondrial genetics MeSH
- Mitochondria genetics MeSH
- DNA Replication MeSH
- Green Fluorescent Proteins chemistry MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The methods of the detection of (1) non-labeled and (2) BrdU-labeled mitochondrial DNA (mtDNA) are described. They are based on the production of singlet oxygen by monovalent copper ions and the subsequent induction of DNA gaps. The ends of interrupted DNA serve as origins for the labeling of mtDNA by DNA polymerase I or they are utilized by exonuclease that degrades DNA strands, unmasking BrdU in BrdU-labeled DNA. Both methods are sensitive approaches without the need of additional enhancement of the signal or the use of highly sensitive optical systems.
References provided by Crossref.org
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- $a The methods of the detection of (1) non-labeled and (2) BrdU-labeled mitochondrial DNA (mtDNA) are described. They are based on the production of singlet oxygen by monovalent copper ions and the subsequent induction of DNA gaps. The ends of interrupted DNA serve as origins for the labeling of mtDNA by DNA polymerase I or they are utilized by exonuclease that degrades DNA strands, unmasking BrdU in BrdU-labeled DNA. Both methods are sensitive approaches without the need of additional enhancement of the signal or the use of highly sensitive optical systems.
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