-
Je něco špatně v tomto záznamu ?
Fully automated two-step assay for detection of metallothionein through magnetic isolation using functionalized γ-Fe2O3 particles
MA. Merlos Rodrigo, L. Krejcova, J. Kudr, N. Cernei, P. Kopel, L. Richtera, A. Moulick, D. Hynek, V. Adam, M. Stiborova, T. Eckschlager, Z. Heger, O. Zitka,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
NV15-28334A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Odkazy
PubMed
27825623
DOI
10.1016/j.jchromb.2016.10.018
Knihovny.cz E-zdroje
- MeSH
- elektroforéza v polyakrylamidovém gelu metody MeSH
- gelová chromatografie metody MeSH
- játra chemie MeSH
- králíci MeSH
- krysa rodu rattus MeSH
- laboratorní automatizace MeSH
- magnetické nanočástice chemie MeSH
- metalothionein analýza izolace a purifikace MeSH
- potkani Wistar MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Metallothioneins (MTs) are involved in heavy metal detoxification in a wide range of living organisms. Currently, it is well known that MTs play substantial role in many pathophysiological processes, including carcinogenesis, and they can serve as diagnostic biomarkers. In order to increase the applicability of MT in cancer diagnostics, an easy-to-use and rapid method for its detection is required. Hence, the aim of this study was to develop a fully automated and high-throughput assay for the estimation of MT levels. Here, we report the optimal conditions for the isolation of MTs from rabbit liver and their characterization using MALDI-TOF MS. In addition, we described a two-step assay, which started with an isolation of the protein using functionalized paramagnetic particles and finished with their electrochemical analysis. The designed easy-to-use, cost-effective, error-free and fully automated procedure for the isolation of MT coupled with a simple analytical detection method can provide a prototype for the construction of a diagnostic instrument, which would be appropriate for the monitoring of carcinogenesis or MT-related chemoresistance of tumors.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17013415
- 003
- CZ-PrNML
- 005
- 20170502094827.0
- 007
- ta
- 008
- 170413s2016 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jchromb.2016.10.018 $2 doi
- 035 __
- $a (PubMed)27825623
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Merlos Rodrigo, Miguel Angel $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 245 10
- $a Fully automated two-step assay for detection of metallothionein through magnetic isolation using functionalized γ-Fe2O3 particles / $c MA. Merlos Rodrigo, L. Krejcova, J. Kudr, N. Cernei, P. Kopel, L. Richtera, A. Moulick, D. Hynek, V. Adam, M. Stiborova, T. Eckschlager, Z. Heger, O. Zitka,
- 520 9_
- $a Metallothioneins (MTs) are involved in heavy metal detoxification in a wide range of living organisms. Currently, it is well known that MTs play substantial role in many pathophysiological processes, including carcinogenesis, and they can serve as diagnostic biomarkers. In order to increase the applicability of MT in cancer diagnostics, an easy-to-use and rapid method for its detection is required. Hence, the aim of this study was to develop a fully automated and high-throughput assay for the estimation of MT levels. Here, we report the optimal conditions for the isolation of MTs from rabbit liver and their characterization using MALDI-TOF MS. In addition, we described a two-step assay, which started with an isolation of the protein using functionalized paramagnetic particles and finished with their electrochemical analysis. The designed easy-to-use, cost-effective, error-free and fully automated procedure for the isolation of MT coupled with a simple analytical detection method can provide a prototype for the construction of a diagnostic instrument, which would be appropriate for the monitoring of carcinogenesis or MT-related chemoresistance of tumors.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a laboratorní automatizace $7 D057205
- 650 _2
- $a gelová chromatografie $x metody $7 D002850
- 650 _2
- $a elektroforéza v polyakrylamidovém gelu $x metody $7 D004591
- 650 _2
- $a játra $x chemie $7 D008099
- 650 _2
- $a magnetické nanočástice $x chemie $7 D058185
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a metalothionein $x analýza $x izolace a purifikace $7 D008668
- 650 _2
- $a králíci $7 D011817
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani Wistar $7 D017208
- 650 _2
- $a spektrometrie hmotnostní - ionizace laserem za účasti matrice $x metody $7 D019032
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Krejcova, Ludmila $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Kudr, Jiri $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Cernei, Natalia $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Kopel, Pavel $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Richtera, Lukas $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Moulick, Amitava $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Hynek, David $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Adam, Vojtěch $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. $7 xx0064599
- 700 1_
- $a Stiborova, Marie $u Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, CZ-128 40 Prague 2, Czech Republic.
- 700 1_
- $a Eckschlager, Tomas $u Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University, and University Hospital Motol, V Uvalu 84, CZ-150 06 Prague 5, Czech Republic.
- 700 1_
- $a Heger, Zbynek $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
- 700 1_
- $a Zitka, Ondrej $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. Electronic address: zitkao@seznam.cz.
- 773 0_
- $w MED00006642 $t Journal of chromatography. B, Analytical technologies in the biomedical and life sciences $x 1873-376X $g Roč. 1039, č. - (2016), s. 17-27
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27825623 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20170413 $b ABA008
- 991 __
- $a 20170502095153 $b ABA008
- 999 __
- $a ok $b bmc $g 1199880 $s 974193
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 1039 $c - $d 17-27 $e 20161014 $i 1873-376X $m Journal of chromatography. B, Analytical technologies in the biomedical and life sciences $n J Chromatogr B Analyt Technol Biomed Life Sci $x MED00006642
- GRA __
- $a NV15-28334A $p MZ0
- LZP __
- $a Pubmed-20170413