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Technical note: Efficient removal of a PCR inhibitory agent (vivianite) found on excavated bones

J. Votrubova-Dubska, D. Vanek, J. Zikmund, O. Mestek, V. Urbanova, H. Brzobohata, P. Brestovansky,

. 2016 ; 261 (-) : 8-13. [pub] 20160115

Language English Country Ireland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc17014095
E-resources Online Full text

NLK ProQuest Central from 1997-02-07 to 2 months ago
Nursing & Allied Health Database (ProQuest) from 1997-02-07 to 2 months ago
Health & Medicine (ProQuest) from 1997-02-07 to 2 months ago

Links

PubMed 26874049
DOI 10.1016/j.forsciint.2015.12.043
Knihovny.cz E-resources

During a rescue excavation in October 2011, archaeologists discovered a mass grave with 10 individuals. The skeletons should belong to victims of the battle of Reichenberg between the Austrian and Prussian armies on April 21, 1757. Several bones of the skeletons were covered with a blue colored encrustation. Initial DNA analysis failed due to strong inhibition. Chemical analysis of the bluish encrustation indicated the presence of the iron phosphate mineral vivianite (Fe3(PO4)2·(H2O)8). This technical note describes a novel procedure for the removal of this inhibitory substance.

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