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Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica
J. Pazourek, K. Šmejkal,
Language English Country Switzerland
Document type Journal Article
E-resources NLK Online Full text
Directory of Open Access Journals from 1997Free Medical Journals from 1997
PubMed Central from 2001
Europe PubMed Central from 2001
ProQuest Central from 1997-01-01
Open Access Digital Library from 1997-01-01
Medline Complete (EBSCOhost) from 2009-03-01
Health & Medicine (ProQuest) from 1997-01-01
Links
PubMed
27834833
DOI
10.3390/molecules21111495
Knihovny.cz E-resources
- MeSH
- Benzyl Alcohols chemistry MeSH
- Fructose chemistry MeSH
- Glucose chemistry MeSH
- Glucosides chemistry MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Scattering, Radiation MeSH
- Temperature MeSH
- Water chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.
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- $a Pazourek, Jiří $u Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech Republic. pazourekj@vfu.cz.
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- $a The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.
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- $a Šmejkal, Karel $u Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech Republic. karel.mejkal@post.cz.
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