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Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions
M. Zovčáková, A. Španová, R. Pantůček, J. Doškař, B. Rittich,
Language English Country United States
Document type Journal Article
- MeSH
- DNA, Bacterial drug effects genetics metabolism MeSH
- Ions chemistry pharmacology MeSH
- Neodymium chemistry pharmacology MeSH
- Plasmids genetics metabolism MeSH
- Staphylococcus aureus genetics MeSH
- Publication type
- Journal Article MeSH
Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd(3+) ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids-closed circular (ccc), open circular (oc), and linear (lin)-produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.
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- $a Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd(3+) ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids-closed circular (ccc), open circular (oc), and linear (lin)-produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.
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