-
Je něco špatně v tomto záznamu ?
Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation
M. Benešová, K. Trejbalová, D. Kučerová, Z. Vernerová, T. Hron, A. Szabó, R. Amouroux, P. Klézl, P. Hajkova, J. Hejnar,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
PubMed
28218476
DOI
10.1002/mc.22638
Knihovny.cz E-zdroje
- MeSH
- 5-methylcytosin analogy a deriváty analýza MeSH
- dioxygenasy analýza genetika MeSH
- DNA vazebné proteiny analýza genetika MeSH
- dospělí MeSH
- lidé MeSH
- metylace DNA * MeSH
- oxygenasy se smíšenou funkcí analýza genetika MeSH
- protoonkogenní proteiny analýza genetika MeSH
- regulace genové exprese u nádorů MeSH
- seminom genetika patologie MeSH
- testikulární nádory genetika patologie MeSH
- testis metabolismus patologie MeSH
- upregulace MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Germ cell tumors and particularly seminomas reflect the epigenomic features of their parental primordial germ cells (PGCs), including genomic DNA hypomethylation and expression of pluripotent cell markers. Because the DNA hypomethylation might be a result of TET dioxygenase activity, we examined expression of TET1-3 enzymes and the level of their product, 5-hydroxymethylcytosine (5hmC), in a panel of histologically characterized seminomas and non-seminomatous germ cell tumors. Expression of TET dioxygenase mRNAs was quantified by real-time PCR. TET1 expression and the level of 5hmC were examined immunohistochemically. Quantitative assessment of 5-methylcytosine (5mC) and 5hmC levels was done by the liquid chromatography-mass spectroscopy technique. We found highly increased expression of TET1 dioxygenase in most seminomas and strong TET1 staining in seminoma cells. Isocitrate dehydrogenase 1 and 2 mutations were not detected, suggesting the enzymatic activity of TET1. The levels of 5mC and 5hmC in seminomas were found decreased in comparison to non-seminomatous germ cell tumors and healthy testicular tissue. We propose that TET1 expression should be studied as a potential marker of seminomas and mixed germ cell tumors and we suggest that elevated expression of TET dioxygenase enzymes is associated with the maintenance of low DNA methylation levels in seminomas. This "anti-methylator" phenotype of seminomas is in contrast to the CpG island methylator phenotype (CIMP) observed in a fraction of tumors of various types.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17031025
- 003
- CZ-PrNML
- 005
- 20171102113231.0
- 007
- ta
- 008
- 171025s2017 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/mc.22638 $2 doi
- 035 __
- $a (PubMed)28218476
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Benešová, Martina $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
- 245 10
- $a Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation / $c M. Benešová, K. Trejbalová, D. Kučerová, Z. Vernerová, T. Hron, A. Szabó, R. Amouroux, P. Klézl, P. Hajkova, J. Hejnar,
- 520 9_
- $a Germ cell tumors and particularly seminomas reflect the epigenomic features of their parental primordial germ cells (PGCs), including genomic DNA hypomethylation and expression of pluripotent cell markers. Because the DNA hypomethylation might be a result of TET dioxygenase activity, we examined expression of TET1-3 enzymes and the level of their product, 5-hydroxymethylcytosine (5hmC), in a panel of histologically characterized seminomas and non-seminomatous germ cell tumors. Expression of TET dioxygenase mRNAs was quantified by real-time PCR. TET1 expression and the level of 5hmC were examined immunohistochemically. Quantitative assessment of 5-methylcytosine (5mC) and 5hmC levels was done by the liquid chromatography-mass spectroscopy technique. We found highly increased expression of TET1 dioxygenase in most seminomas and strong TET1 staining in seminoma cells. Isocitrate dehydrogenase 1 and 2 mutations were not detected, suggesting the enzymatic activity of TET1. The levels of 5mC and 5hmC in seminomas were found decreased in comparison to non-seminomatous germ cell tumors and healthy testicular tissue. We propose that TET1 expression should be studied as a potential marker of seminomas and mixed germ cell tumors and we suggest that elevated expression of TET dioxygenase enzymes is associated with the maintenance of low DNA methylation levels in seminomas. This "anti-methylator" phenotype of seminomas is in contrast to the CpG island methylator phenotype (CIMP) observed in a fraction of tumors of various types.
- 650 _2
- $a 5-methylcytosin $x analogy a deriváty $x analýza $7 D044503
- 650 _2
- $a dospělí $7 D000328
- 650 12
- $a metylace DNA $7 D019175
- 650 _2
- $a DNA vazebné proteiny $x analýza $x genetika $7 D004268
- 650 _2
- $a dioxygenasy $x analýza $x genetika $7 D049308
- 650 _2
- $a regulace genové exprese u nádorů $7 D015972
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a oxygenasy se smíšenou funkcí $x analýza $x genetika $7 D006899
- 650 _2
- $a protoonkogenní proteiny $x analýza $x genetika $7 D011518
- 650 _2
- $a seminom $x genetika $x patologie $7 D018239
- 650 _2
- $a testikulární nádory $x genetika $x patologie $7 D013736
- 650 _2
- $a testis $x metabolismus $x patologie $7 D013737
- 650 _2
- $a upregulace $7 D015854
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Trejbalová, Kateřina $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
- 700 1_
- $a Kučerová, Dana $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
- 700 1_
- $a Vernerová, Zdenka $u Department of Pathology, Third Faculty of Medicine, Charles University in Prague, Ruska 87, CZ-10000, Prague 10, Czech Republic.
- 700 1_
- $a Hron, Tomáš $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
- 700 1_
- $a Szabó, Arpád $u Department of Pathology, Third Faculty of Medicine, Charles University in Prague, Ruska 87, CZ-10000, Prague 10, Czech Republic.
- 700 1_
- $a Amouroux, Rachel $u MRC London Institute of Medical Sciences, London, UK and Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, UK. $7 gn_A_00005734
- 700 1_
- $a Klézl, Petr $u Department of Urology, Third Faculty of Medicine, Charles University in Prague, Ruska 87, CZ-10000, Prague 10, Czech Republic.
- 700 1_
- $a Hajkova, Petra $u MRC London Institute of Medical Sciences, London, UK and Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, UK.
- 700 1_
- $a Hejnar, Jiří $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
- 773 0_
- $w MED00188779 $t Molecular carcinogenesis $x 1098-2744 $g Roč. 56, č. 8 (2017), s. 1837-1850
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28218476 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20171102113324 $b ABA008
- 999 __
- $a ok $b bmc $g 1254618 $s 992052
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 56 $c 8 $d 1837-1850 $e 20170524 $i 1098-2744 $m Molecular carcinogenesis $n Mol Carcinog $x MED00188779
- LZP __
- $a Pubmed-20171025