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WNT5A: a motility-promoting factor in Hodgkin lymphoma
F. Linke, S. Zaunig, MM. Nietert, F. von Bonin, S. Lutz, C. Dullin, P. Janovská, T. Beissbarth, F. Alves, W. Klapper, V. Bryja, T. Pukrop, L. Trümper, J. Wilting, D. Kube,
Language English Country England, Great Britain
Document type Journal Article
NLK
ProQuest Central
from 1997-01-01 to 2017-12-31
Open Access Digital Library
from 1997-01-01
Health & Medicine (ProQuest)
from 1997-01-01 to 2017-12-31
Public Health Database (ProQuest)
from 1997-01-01 to 2019-12-31
PubMed
27270428
DOI
10.1038/onc.2016.183
Knihovny.cz E-resources
- MeSH
- Models, Biological MeSH
- Biopsy MeSH
- Cell Adhesion genetics MeSH
- Porcupines MeSH
- Gene Expression MeSH
- Frizzled Receptors metabolism MeSH
- Hodgkin Disease diagnostic imaging genetics metabolism pathology MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Tomography, X-Ray Computed MeSH
- Cell Movement genetics MeSH
- Cell Proliferation MeSH
- Dishevelled Proteins metabolism MeSH
- Wnt-5a Protein genetics metabolism MeSH
- rhoA GTP-Binding Protein metabolism MeSH
- Signal Transduction MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Classical Hodgkin lymphoma (cHL) has a typical clinical manifestation, with dissemination involving functionally neighboring lymph nodes. The factors involved in the spread of lymphoma cells are poorly understood. Here we show that cHL cell lines migrate with higher rates compared with non-Hodgkin lymphoma cell lines. cHL cell migration, invasion and adhesion depend on autocrine WNT signaling as revealed by the inhibition of WNT secretion with the porcupine inhibitors Wnt-C59/IWP-2, but did not affect cell proliferation. While application of recombinant WNT5A or WNT5A overexpression stimulates HL cell migration, neither WNT10A, WNT10B nor WNT16 did so. Time-lapse studies revealed an amoeboid type of cell migration modulated by WNT5A. Reduced migration distances and velocity of cHL cells, as well as altered movement patterns, were observed using porcupine inhibitor or WNT5A antagonist. Knockdown of Frizzled5 and Dishevelled3 disrupted the WNT5A-mediated RHOA activation and cell migration. Overexpression of DVL3-K435M or inhibition of ROCK (Rho-associated protein kinase) by Y-27632/H1152P disrupted cHL cell migration. In addition to these mechanistic insights into the role of WNT5A in vitro, global gene expression data revealed an increased WNT5A expression in primary HL cells in comparison with normal B-cell subsets and other lymphomas. Furthermore, the activity of both porcupine and WNT5A in cHL cells had an impact on lymphoma development in the chick chorionallantoic membrane assay. Massive bleeding of these lymphomas was significantly reduced after inhibition of WNT secretion by Wnt-C59. Therefore, a model is proposed where WNT signaling has an important role in regulating tumor-promoting processes.
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