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Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile
Y. Lustig, H. Zelena, G. Venturi, M. Van Esbroeck, C. Rothe, C. Perret, R. Koren, S. Katz-Likvornik, E. Mendelson, E. Schwartz,
Language English Country United States
Document type Evaluation Study, Journal Article
NLK
Free Medical Journals
from 1975 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1975 to 1 year ago
Europe PubMed Central
from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
28381608
DOI
10.1128/jcm.00346-17
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Travel MeSH
- Child MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay methods MeSH
- Communicable Diseases, Imported diagnosis MeSH
- Immunoglobulin G blood MeSH
- Immunoglobulin M blood MeSH
- Zika Virus Infection diagnosis MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Child, Preschool MeSH
- Antibodies, Viral blood MeSH
- Aged MeSH
- Sensitivity and Specificity MeSH
- Serologic Tests methods MeSH
- Antibody Formation MeSH
- Viral Nonstructural Proteins immunology MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Geographicals
- Chile MeSH
- Europe MeSH
Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.
Central Virology Laboratory Ministry of Health Ramat Gan Israel
Department of Clinical Sciences Institute of Tropical Medicine Antwerp Belgium
Istituto Superiore di Sanità Rome Italy
Laboratory of Virology School of Medicine Pontificia Universidad Catolica de Chile Santiago Chile
References provided by Crossref.org
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