Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization

M. Frolikova, P. Manaskova-Postlerova, J. Cerny, J. Jankovicova, O. Simonik, A. Pohlova, P. Secova, J. Antalikova, K. Dvorakova-Hortova,

. 2018 ; 19 (4) : . [pub] 20180419

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033145

Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm⁻egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9/CD81 double null female mice. In this study, the putative mechanism of CD9/CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9/CD81 localization visualised in detail by super-resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosome reaction in human sperm, only CD9 is relocated, compared to the relocation of both proteins in mice. The structural modelling of CD9 and CD81 homologous and possibly heterologous network formation was used to propose their lateral Cis as well as Trans interactions within the sperm membrane and during sperm⁻egg membrane fusion.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033145
003      
CZ-PrNML
005      
20181010124858.0
007      
ta
008      
181008s2018 sz f 000 00|eng||
009      
AR
024    7_
$a 10.3390/ijms19041236 $2 doi
035    __
$a (PubMed)29671763
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Frolikova, Michaela $u Group of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. Michaela.Frolikova@ibt.cas.cz.
245    10
$a CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization / $c M. Frolikova, P. Manaskova-Postlerova, J. Cerny, J. Jankovicova, O. Simonik, A. Pohlova, P. Secova, J. Antalikova, K. Dvorakova-Hortova,
520    9_
$a Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm⁻egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9/CD81 double null female mice. In this study, the putative mechanism of CD9/CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9/CD81 localization visualised in detail by super-resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosome reaction in human sperm, only CD9 is relocated, compared to the relocation of both proteins in mice. The structural modelling of CD9 and CD81 homologous and possibly heterologous network formation was used to propose their lateral Cis as well as Trans interactions within the sperm membrane and during sperm⁻egg membrane fusion.
650    12
$a akrozomální reakce $7 D020101
650    _2
$a zvířata $7 D000818
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a fertilizace $7 D005306
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a fúze membrán $7 D008561
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a molekulární modely $7 D008958
650    _2
$a mapy interakcí proteinů $7 D060066
650    12
$a kapacitace spermií $7 D013075
650    _2
$a spermie $x cytologie $x metabolismus $x ultrastruktura $7 D013094
650    _2
$a antigeny CD81 $x analýza $x metabolismus $7 D060225
650    _2
$a antigeny CD9 $x analýza $x metabolismus $7 D060245
655    _2
$a časopisecké články $7 D016428
700    1_
$a Manaskova-Postlerova, Pavla $u Group of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. pavla.postlerova@ibt.cas.cz. Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, Kamycka 129, 165 00 Prague, Czech Republic. pavla.postlerova@ibt.cas.cz.
700    1_
$a Cerny, Jiri $u Laboratory of Structural Bioinformatics of Proteins, Institute of Biotechnology Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. Jiri.Cerny@ibt.cas.cz.
700    1_
$a Jankovicova, Jana $u Laboratory of Reproductive Physiology, Institute of Animal Biochemistry and Genetics Centre of Biosciences Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia. jana.jankovicova@savba.sk.
700    1_
$a Simonik, Ondrej $u Group of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. Ondrej.Simonik@ibt.cas.cz. Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, Kamycka 129, 165 00 Prague, Czech Republic. Ondrej.Simonik@ibt.cas.cz.
700    1_
$a Pohlova, Alzbeta $u Group of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. Alzbeta.Pohlova@ibt.cas.cz. Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 43 Prague, Czech Republic. Alzbeta.Pohlova@ibt.cas.cz.
700    1_
$a Secova, Petra $u Laboratory of Reproductive Physiology, Institute of Animal Biochemistry and Genetics Centre of Biosciences Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia. petra.secova@savba.sk.
700    1_
$a Antalikova, Jana $u Laboratory of Reproductive Physiology, Institute of Animal Biochemistry and Genetics Centre of Biosciences Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia. Jana.Antalikova@savba.sk.
700    1_
$a Dvorakova-Hortova, Katerina $u Group of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, v.v.i., BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic. katerina.hortova@ibt.cas.cz. Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague, Czech Republic. katerina.hortova@ibt.cas.cz.
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 19, č. 4 (2018)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29671763 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181010125348 $b ABA008
999    __
$a ok $b bmc $g 1340821 $s 1030139
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 19 $c 4 $e 20180419 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace