• Je něco špatně v tomto záznamu ?

Influence of insemination time on the fertility of sex sorted frozen-thawed Y-sperm in red deer

L. Anel-López, O. Garcia-Álvarez, T. Tarantini, D. Del Olmo, JA. Ortiz, S. Ledda, EA. Martinez, AJ. Soler, J. Roca, MR. Fernández Santos, JM. Vázquez, I. Parrilla, JJ. Garde,

. 2018 ; 113 (-) : 171-175. [pub] 20180309

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000866

The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon® PMSG Serum Gonadotrophin) on day 12, upon CIDR removal. They were then inseminated with one of each sperm treatment, at the following post-eCG intervals: I_1, 55:01-55:30 h; I_2, 55:31-56:00 h; I_3, 56:01-56:30 h; or, I_4, 56:31-57:00 h. Pregnancy rates were assessed at parturition. Average pregnancy rates were highest (P < 0.05) for Conventional samples (77.6%), but similar between YSS (49.8%) and Control sorted (51.3%) samples. However, when insemination interval was taken into account, pregnancy rates within the YSS group, pregnancy rates were 80 and 83.1% for I_1 and I_2, respectively were obtained. Notably, these rates were similar (P > 0.05) to the average pregnancy rates obtained with Conventional samples (77.6%). As expected, YSS sperm yielded 94% male offspring contrasting with the 57% males obtained with Conventional and Control sorted samples. Our findings support the importance of developing specific insemination timing protocols to improve pregnancy rates when using frozen-thawed sex-sorted sperm. These findings provide the foundation for further investigations in order to determine why the YSS sperm are able to fertilize the oocyte in a shorter period of time than the conventional samples.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000866
003      
CZ-PrNML
005      
20190121105601.0
007      
ta
008      
190107s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.theriogenology.2018.03.005 $2 doi
035    __
$a (PubMed)29547733
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Anel-López, Luis $u ITRA-ULE, Departamento de Medicina, Cirugía y Anatomía Veterinaria, University of León, Spain.
245    10
$a Influence of insemination time on the fertility of sex sorted frozen-thawed Y-sperm in red deer / $c L. Anel-López, O. Garcia-Álvarez, T. Tarantini, D. Del Olmo, JA. Ortiz, S. Ledda, EA. Martinez, AJ. Soler, J. Roca, MR. Fernández Santos, JM. Vázquez, I. Parrilla, JJ. Garde,
520    9_
$a The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon® PMSG Serum Gonadotrophin) on day 12, upon CIDR removal. They were then inseminated with one of each sperm treatment, at the following post-eCG intervals: I_1, 55:01-55:30 h; I_2, 55:31-56:00 h; I_3, 56:01-56:30 h; or, I_4, 56:31-57:00 h. Pregnancy rates were assessed at parturition. Average pregnancy rates were highest (P < 0.05) for Conventional samples (77.6%), but similar between YSS (49.8%) and Control sorted (51.3%) samples. However, when insemination interval was taken into account, pregnancy rates within the YSS group, pregnancy rates were 80 and 83.1% for I_1 and I_2, respectively were obtained. Notably, these rates were similar (P > 0.05) to the average pregnancy rates obtained with Conventional samples (77.6%). As expected, YSS sperm yielded 94% male offspring contrasting with the 57% males obtained with Conventional and Control sorted samples. Our findings support the importance of developing specific insemination timing protocols to improve pregnancy rates when using frozen-thawed sex-sorted sperm. These findings provide the foundation for further investigations in order to determine why the YSS sperm are able to fertilize the oocyte in a shorter period of time than the conventional samples.
650    _2
$a zvířata $7 D000818
650    _2
$a kryoprezervace $x veterinární $7 D015925
650    _2
$a vysoká zvěř $x fyziologie $7 D003670
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a fertilita $x fyziologie $7 D005298
650    12
$a zmrazování $7 D005615
650    _2
$a umělá inseminace $x veterinární $7 D007315
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a těhotenství $7 D011247
650    _2
$a uchování spermatu $x veterinární $7 D012662
650    _2
$a předvýběr pohlaví $x veterinární $7 D012743
650    _2
$a spermie $x fyziologie $7 D013094
655    _2
$a časopisecké články $7 D016428
700    1_
$a Garcia-Álvarez, Olga $u Biomedical Center, School of Medicine in Pilsen, Charles University in Prague, alej Svobody 1655/76, 323 00 Pilsen, Czech Republic; SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain.
700    1_
$a Tarantini, Tatiana $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Del Olmo, David $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Ortiz, Jose Antonio $u Medianilla S.L. Finca Las Lomas, Vejer de la Frontera, Cádiz, Spain.
700    1_
$a Ledda, Sergio $u Dipartamento di Medicina Veterinaria, Universita degli Studi di Sassari, Italy.
700    1_
$a Martinez, Emilio Arsenio $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Soler, Ana J $u SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain.
700    1_
$a Roca, Jordi $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Fernández Santos, María R $u SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain.
700    1_
$a Vázquez, Juan Maria $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Parrilla, Inmaculada $u Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
700    1_
$a Garde, Jose Julián $u SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain. Electronic address: Julian.Garde@uclm.es.
773    0_
$w MED00004512 $t Theriogenology $x 1879-3231 $g Roč. 113, č. - (2018), s. 171-175
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29547733 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190121105819 $b ABA008
999    __
$a ok $b bmc $g 1363938 $s 1038989
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 113 $c - $d 171-175 $e 20180309 $i 1879-3231 $m Theriogenology $n Theriogenology $x MED00004512
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace