Detail
Článek
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

Z. Tulpová, MC. Luo, H. Toegelová, P. Visendi, S. Hayashi, P. Vojta, E. Paux, A. Kilian, M. Abrouk, J. Bartoš, M. Hajdúch, J. Batley, D. Edwards, J. Doležel, H. Šimková,

. 2019 ; 48 (-) : 12-19. [pub] 20180308

Jazyk angličtina Země Nizozemsko

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000877

Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding. Physical maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of bread wheat and to assist map-based gene cloning. As a part of a joint effort coordinated by the International Wheat Genome Sequencing Consortium, we have constructed a BAC-based physical map of bread wheat chromosome arm 7DS consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid map and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This map integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up gene cloning in wheat. The process of physical map assembly included the integration of the 7DS physical map with a whole-genome physical map of Aegilops tauschii and a 7DS Bionano genome map, which together enabled efficient scaffolding of physical-map contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of bread wheat and its ancestor at BAC-contig level and revealed a reconstructed region in the 7DS pericentromere.

000      
00000naa a2200000 a 4500
001      
bmc19000877
003      
CZ-PrNML
005      
20200121144623.0
007      
ta
008      
190107s2019 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.nbt.2018.03.003 $2 doi
035    __
$a (PubMed)29526810
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Tulpová, Zuzana $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: tulpova@ueb.cas.cz.
245    10
$a Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies / $c Z. Tulpová, MC. Luo, H. Toegelová, P. Visendi, S. Hayashi, P. Vojta, E. Paux, A. Kilian, M. Abrouk, J. Bartoš, M. Hajdúch, J. Batley, D. Edwards, J. Doležel, H. Šimková,
520    9_
$a Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding. Physical maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of bread wheat and to assist map-based gene cloning. As a part of a joint effort coordinated by the International Wheat Genome Sequencing Consortium, we have constructed a BAC-based physical map of bread wheat chromosome arm 7DS consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid map and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This map integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up gene cloning in wheat. The process of physical map assembly included the integration of the 7DS physical map with a whole-genome physical map of Aegilops tauschii and a 7DS Bionano genome map, which together enabled efficient scaffolding of physical-map contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of bread wheat and its ancestor at BAC-contig level and revealed a reconstructed region in the 7DS pericentromere.
650    _2
$a Aegilops $x genetika $7 D000078065
650    _2
$a centromera $x genetika $7 D002503
650    _2
$a umělé bakteriální chromozomy $x genetika $7 D022202
650    _2
$a chromozomy rostlin $x genetika $7 D032461
650    _2
$a klonování DNA $7 D003001
650    _2
$a rostlinné geny $7 D017343
650    _2
$a genom rostlinný $7 D018745
650    _2
$a hybridizace genetická $7 D006824
650    _2
$a fyzikální mapování chromozomů $x metody $7 D020161
650    _2
$a šlechtění rostlin $7 D000069600
650    _2
$a pšenice $x genetika $7 D014908
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
700    1_
$a Luo, Ming-Cheng $u Department of Plant Sciences, University of California, Davis, USA. Electronic address: mcluo@ucdavis.edu.
700    1_
$a Toegelová, Helena $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: toegelova@ueb.cas.cz.
700    1_
$a Visendi, Paul $u Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK. Electronic address: P.Muhindira@greenwich.ac.uk.
700    1_
$a Hayashi, Satomi $u Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Queensland, Australia. Electronic address: satomi.hayashi@qut.edu.au.
700    1_
$a Vojta, Petr $u Laboratoty of Experimental Medicine, Institute of Molecular and Translational Medicine, Hněvotínská 5, 779 00 Olomouc, Czech Republic. Electronic address: petr.vojta@upol.cz.
700    1_
$a Paux, Etienne $u GDEC, INRA, Université Clermont Auvergne, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France. Electronic address: etienne.paux@inra.fr.
700    1_
$a Kilian, Andrzej $u Diversity Arrays Technology Pty Ltd, University of Canberra, Bruce, Australia. Electronic address: a.kilian@diversityarrays.com.
700    1_
$a Abrouk, Michaël $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: abrouk@ueb.cas.cz.
700    1_
$a Bartoš, Jan $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: bartos@ueb.cas.cz.
700    1_
$a Hajdúch, Marián $u Laboratoty of Experimental Medicine, Institute of Molecular and Translational Medicine, Hněvotínská 5, 779 00 Olomouc, Czech Republic. Electronic address: marian.hajduch@upol.cz.
700    1_
$a Batley, Jacqueline $u School of Biological Sciences and Institute of Agriculture, University of Western Australia, Crawley, WA, Australia. Electronic address: Jacqueline.batley@uwa.edu.au.
700    1_
$a Edwards, David $u School of Biological Sciences and Institute of Agriculture, University of Western Australia, Crawley, WA, Australia. Electronic address: Dave.Edwards@uwa.edu.au.
700    1_
$a Doležel, Jaroslav $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: dolezel@ueb.cas.cz.
700    1_
$a Šimková, Hana $u Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 783 71 Olomouc, Czech Republic. Electronic address: simkovah@ueb.cas.cz.
773    0_
$w MED00167291 $t New biotechnology $x 1876-4347 $g Roč. 48, č. - (2019), s. 12-19
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29526810 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20200121145000 $b ABA008
999    __
$a ok $b bmc $g 1364863 $s 1039000
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 48 $c - $d 12-19 $e 20180308 $i 1876-4347 $m New biotechnology $n N Biotechnol $x MED00167291
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace