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Ultrastructural and Molecular Analysis of Ribose-Induced Glycated Reconstructed Human Skin
R. Balansin Rigon, S. Kaessmeyer, C. Wolff, C. Hausmann, N. Zhang, M. Sochorová, A. Kováčik, R. Haag, K. Vávrová, M. Ulrich, M. Schäfer-Korting, C. Zoschke,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Free Medical Journals od 2000
Freely Accessible Science Journals od 2000
PubMed Central od 2007
Europe PubMed Central od 2007
ProQuest Central od 2000-03-01
Open Access Digital Library od 2000-01-01
Open Access Digital Library od 2007-01-01
Health & Medicine (ProQuest) od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources od 2000
Odkazy
PubMed
30413126
DOI
10.3390/ijms19113521
Knihovny.cz E-zdroje
- MeSH
- bazální membrána účinky léků ultrastruktura MeSH
- buněčná diferenciace účinky léků MeSH
- epidermis účinky léků ultrastruktura MeSH
- fibroblasty účinky léků ultrastruktura MeSH
- keratinocyty účinky léků ultrastruktura MeSH
- kůže účinky léků ultrastruktura MeSH
- lidé MeSH
- produkty pokročilé glykace metabolismus MeSH
- ribosa farmakologie MeSH
- škára účinky léků ultrastruktura MeSH
- stárnutí kůže účinky léků MeSH
- transmisní elektronová mikroskopie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Aging depicts one of the major challenges in pharmacology owing to its complexity and heterogeneity. Thereby, advanced glycated end-products modify extracellular matrix proteins, but the consequences on the skin barrier function remain heavily understudied. Herein, we utilized transmission electron microscopy for the ultrastructural analysis of ribose-induced glycated reconstructed human skin (RHS). Molecular and functional insights substantiated the ultrastructural characterization and proved the relevance of glycated RHS beyond skin aging. In particular, electron microscopy mapped the accumulation and altered spatial orientation of fibrils and filaments in the dermal compartment of glycated RHS. Moreover, the epidermal basement membrane appeared thicker in glycated than in non-glycated RHS, but electron microscopy identified longitudinal clusters of the finest collagen fibrils instead of real thickening. The stratum granulosum contained more cell layers, the morphology of keratohyalin granules decidedly differed, and the stratum corneum lipid order increased in ribose-induced glycated RHS, while the skin barrier function was almost not affected. In conclusion, dermal advanced glycated end-products markedly changed the epidermal morphology, underlining the importance of matrix⁻cell interactions. The phenotype of ribose-induced glycated RHS emulated aged skin in the dermis, while the two to three times increased thickness of the stratum granulosum resembled poorer cornification.
Collegium Medicum Berlin Luisenstr 54 10117 Berlin Germany
Institute of Chemistry and Biochemistry Freie Universität Berlin Takustr 3 14195 Berlin Germany
Institute of Pharmacy Freie Universität Berlin Königin Luise Str 2 4 14195 Berlin Germany
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- $a Balansin Rigon, Roberta $u Institute of Pharmacy (Pharmacology & Toxicology), Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195 Berlin, Germany. roberta_rigon@yahoo.com.br.
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- $a Ultrastructural and Molecular Analysis of Ribose-Induced Glycated Reconstructed Human Skin / $c R. Balansin Rigon, S. Kaessmeyer, C. Wolff, C. Hausmann, N. Zhang, M. Sochorová, A. Kováčik, R. Haag, K. Vávrová, M. Ulrich, M. Schäfer-Korting, C. Zoschke,
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- $a Aging depicts one of the major challenges in pharmacology owing to its complexity and heterogeneity. Thereby, advanced glycated end-products modify extracellular matrix proteins, but the consequences on the skin barrier function remain heavily understudied. Herein, we utilized transmission electron microscopy for the ultrastructural analysis of ribose-induced glycated reconstructed human skin (RHS). Molecular and functional insights substantiated the ultrastructural characterization and proved the relevance of glycated RHS beyond skin aging. In particular, electron microscopy mapped the accumulation and altered spatial orientation of fibrils and filaments in the dermal compartment of glycated RHS. Moreover, the epidermal basement membrane appeared thicker in glycated than in non-glycated RHS, but electron microscopy identified longitudinal clusters of the finest collagen fibrils instead of real thickening. The stratum granulosum contained more cell layers, the morphology of keratohyalin granules decidedly differed, and the stratum corneum lipid order increased in ribose-induced glycated RHS, while the skin barrier function was almost not affected. In conclusion, dermal advanced glycated end-products markedly changed the epidermal morphology, underlining the importance of matrix⁻cell interactions. The phenotype of ribose-induced glycated RHS emulated aged skin in the dermis, while the two to three times increased thickness of the stratum granulosum resembled poorer cornification.
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