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TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei

D. Faktorová, A. Bär, H. Hashimi, K. McKenney, A. Horák, A. Schnaufer, MAT. Rubio, JD. Alfonzo, J. Lukeš,

. 2018 ; 225 (-) : 84-93. [pub] 20180921

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19012341

Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.

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$a Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.
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$a Bär, Anita $u Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
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$a Hashimi, Hassan $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
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$a McKenney, Katherine $u Department of Microbiology, The Ohio State University, Columbus, OH, USA; The Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
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$a Horák, Aleš $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
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$a Schnaufer, Achim $u Centre of Immunity, Infection and Evolution and Institute of Immunology & Infection Research, University of Edinburgh, Edinburgh, United Kingdom.
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$a Rubio, Mary Anne T $u Department of Microbiology, The Ohio State University, Columbus, OH, USA; The Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
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$a Alfonzo, Juan D $u Department of Microbiology, The Ohio State University, Columbus, OH, USA; The Center for RNA Biology, The Ohio State University, Columbus, OH, USA; The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA.
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$a Lukeš, Julius $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005, České Budějovice, Czech Republic.
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