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Fast enantioseparation of indole phytoalexins in additive free supercritical fluid chromatography
O. Kozlov, K. Kalíková, T. Gondová, M. Budovská, A. Salayová, E. Tesařová,
Language English Country Netherlands
Document type Journal Article
- MeSH
- Amylose chemistry MeSH
- Cellulose chemistry MeSH
- Chemistry Techniques, Analytical methods MeSH
- Indoles isolation & purification MeSH
- Silicon Dioxide chemistry MeSH
- Polysaccharides chemistry MeSH
- Solvents chemistry MeSH
- Sesquiterpenes isolation & purification MeSH
- Stereoisomerism MeSH
- Chromatography, Supercritical Fluid * MeSH
- Publication type
- Journal Article MeSH
A series of chiral indole phytoalexins with potential anticancer and antimicrobial activity were enantioseparated in supercritical fluid chromatography. Two polysaccharide-based chiral stationary phases composed of tris-(3,5-dimethylphenylcarbamate) derivatives of amylose or cellulose coated on 2.5 μm silica particles were successfully used. The influences of the polysaccharide backbone, co-solvent type and co-solvent content in the mobile phase on retention, enantioselectivity and enantioresolution of indole phytoalexins were investigated. Fast baseline separations were achieved for 26 from 27 tested compounds. Amylose-based chiral stationary phase provided higher number of baseline resolutions of the indole phytoalexins than the cellulose-based one. However, certain complementary enantioresolution results towards the studied compounds were observed between the investigated columns. The relationship between structure of the indole phytoalexins and their chromatographic behavior in supercritical fluid chromatography was discussed.
References provided by Crossref.org
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- $a Kozlov, Oleksandr $u Department of Analytical Chemistry, Faculty of Science, P. J. Šafárik University, Moyzesova 11, 040 01 Košice, Slovak Republic.
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- $a A series of chiral indole phytoalexins with potential anticancer and antimicrobial activity were enantioseparated in supercritical fluid chromatography. Two polysaccharide-based chiral stationary phases composed of tris-(3,5-dimethylphenylcarbamate) derivatives of amylose or cellulose coated on 2.5 μm silica particles were successfully used. The influences of the polysaccharide backbone, co-solvent type and co-solvent content in the mobile phase on retention, enantioselectivity and enantioresolution of indole phytoalexins were investigated. Fast baseline separations were achieved for 26 from 27 tested compounds. Amylose-based chiral stationary phase provided higher number of baseline resolutions of the indole phytoalexins than the cellulose-based one. However, certain complementary enantioresolution results towards the studied compounds were observed between the investigated columns. The relationship between structure of the indole phytoalexins and their chromatographic behavior in supercritical fluid chromatography was discussed.
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