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Chromosome-based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides

BA. Akpinar, S. Biyiklioglu, B. Alptekin, M. Havránková, J. Vrána, J. Doležel, A. Distelfeld, P. Hernandez, IWGSC, H. Budak,

. 2018 ; 16 (12) : 2077-2087. [pub] 20180613

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19028443

Grantová podpora
Winifred-Asbjornson - International
P501/12/G090 Czech Science Foundation - International
LO1204 Ministry of Education, Youth and Sports of the Czech Republic - International
National Program of Sustainability I - International
AGL2016-77149-C2-1-P MINECO - International
CGL2016-79790-P MINECO - International
P12-AGR-0482 Junta de Andalucia - International

Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of wheat. We performed chromosome-based survey sequencing of the 14 chromosomes, examining repetitive sequences, protein-coding genes, miRNA/target pairs and tRNA genes, as well as syntenic relationships with related grasses. We found considerable differences in the content and distribution of repetitive sequences between the A and B subgenomes. The gene contents of individual chromosomes varied widely, not necessarily correlating with chromosome size. We catalogued candidate agronomically important loci, along with new alleles and flanking sequences that can be used to design exome sequencing. Syntenic relationships and virtual gene orders revealed several small-scale evolutionary rearrangements, in addition to providing evidence for the 4AL-5AL-7BS translocation in wild emmer wheat. Chromosome-based sequence assemblies contained five novel miRNA families, among 59 families putatively encoded in the entire genome which provide insight into the domestication of wheat and an overview of the genome content and organization.

Citace poskytuje Crossref.org

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