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Highly variable vancomycin-resistant enterococci in the north-eastern part of the Czech Republic
V. Oravcova, M. Kolar, I. Literak,
Language English Country England, Great Britain
Document type Journal Article
Grant support
NV18-05-00340
Ministerstvo Zdravotnictví Ceské Republiky
CZ.1.05/1.1.00/02.0068
Central European Institute of Technology
PubMed
30697771
DOI
10.1111/lam.13121
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacterial Proteins genetics MeSH
- Enterococcus faecalis drug effects genetics isolation & purification MeSH
- Enterococcus faecium drug effects genetics isolation & purification MeSH
- Vancomycin-Resistant Enterococci classification genetics isolation & purification MeSH
- Gram-Positive Bacterial Infections epidemiology microbiology MeSH
- Humans MeSH
- Plasmids genetics MeSH
- Vancomycin Resistance genetics MeSH
- Toxin-Antitoxin Systems genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
Vancomycin-resistant enterococci (VRE) are nosocomial pathogens of increasing medical importance. This study involved 121 VRE selectively obtained from a representative set of 1464 samples collected from various sources in the north-eastern part of the Czech Republic. In total, 119 VRE belonged to Enterococcus faecium and two to Enterococcus faecalis. All isolates of E. faecium were resistant to at least three antibiotic classes. The resistance genes vanA, erm(B), tet(M), tet(L), aac(3)-IIIa and aac(6')-aph(2'') were detected. We assigned the E. faecium to sequence types ST5, ST18, ST38, ST64, ST92, ST273, ST549 and ST640. In E. faecium isolates, we identified the presence of replicases rep20pLG1 , rep2pRE25 , rep17pRUM , rep21pVEF1/2 and rep14pRI1 , as well as relaxases relpEF1 , relpLG1 , relpCIZ2 , relpRE25 and relpRUM . The presence of the toxin-antitoxin system axe-txe was detected mainly among isolates of hospital origin. The A and D types of transposon Tn1546 were those occurring most frequently. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first extensive study of vancomycin-resistant enterococci of diverse origin in a single well-defined area of the Czech Republic. The isolates were investigated for their antibiotic resistance, epidemiological characteristics and plasmid characteristics. Based on the results obtained, we can make assumptions as to the ways that vancomycin resistance is disseminated throughout the environment including humans and animals.
References provided by Crossref.org
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