-
Something wrong with this record ?
FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
PR. Nicovich, JM. Kwiatek, Y. Ma, A. Benda, K. Gaus,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Cell Press Free Archives
from 1960-01-01 to 1 year ago
Free Medical Journals
from 1960 to 1 year ago
Freely Accessible Science Journals
from 1960 to 12 months ago
PubMed Central
from 1960 to 1 year ago
Europe PubMed Central
from 1960 to 1 year ago
Open Access Digital Library
from 1960-09-01
- MeSH
- Benzoxazines chemistry MeSH
- Cell Membrane chemistry MeSH
- Chlorocebus aethiops MeSH
- COS Cells MeSH
- Spectrometry, Fluorescence MeSH
- Quaternary Ammonium Compounds chemistry MeSH
- Membrane Lipids chemistry MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The coexistence of lipid domains with different degrees of lipid packing in the plasma membrane of mammalian cells has been postulated, but direct evidence has so far been challenging to obtain because of the small size and short lifetime of these domains in live cells. Here, we use fluorescence spectral correlation spectroscopy in conjunction with a probe sensitive to the membrane environment to quantify spectral fluctuations associated with dynamics of membrane domains in live cells. With this method, we show that membrane domains are present in live COS-7 cells and have a lifetime lower bound of 5.90 and 14.69 ms for the ordered and disordered phases, respectively. Comparisons to simulations indicate that the underlying mechanism of these fluctuations is complex but qualitatively described by a combination of dye diffusion between membrane domains as well as the motion of domains within the membrane.
Allen Institute for Brain Science Seattle Washington
Imaging Methods Core Facility at BIOCEV Faculty of Sciences Charles University Vestec Czech Republic
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19035177
- 003
- CZ-PrNML
- 005
- 20191011085157.0
- 007
- ta
- 008
- 191007s2018 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.bpj.2018.04.050 $2 doi
- 035 __
- $a (PubMed)29925022
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Nicovich, Philip R $u EMBL Australia Node in Single Molecule Science, School of Medical Sciences, Sydney, New South Wales, Australia; ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia; Allen Institute for Brain Science, Seattle, Washington. Electronic address: rustyn@alleninstitute.org.
- 245 10
- $a FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells / $c PR. Nicovich, JM. Kwiatek, Y. Ma, A. Benda, K. Gaus,
- 520 9_
- $a The coexistence of lipid domains with different degrees of lipid packing in the plasma membrane of mammalian cells has been postulated, but direct evidence has so far been challenging to obtain because of the small size and short lifetime of these domains in live cells. Here, we use fluorescence spectral correlation spectroscopy in conjunction with a probe sensitive to the membrane environment to quantify spectral fluctuations associated with dynamics of membrane domains in live cells. With this method, we show that membrane domains are present in live COS-7 cells and have a lifetime lower bound of 5.90 and 14.69 ms for the ordered and disordered phases, respectively. Comparisons to simulations indicate that the underlying mechanism of these fluctuations is complex but qualitatively described by a combination of dye diffusion between membrane domains as well as the motion of domains within the membrane.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a benzoxaziny $x chemie $7 D048588
- 650 _2
- $a COS buňky $7 D019556
- 650 _2
- $a buněčná membrána $x chemie $7 D002462
- 650 _2
- $a viabilita buněk $7 D002470
- 650 _2
- $a Cercopithecus aethiops $7 D002522
- 650 _2
- $a membránové lipidy $x chemie $7 D008563
- 650 _2
- $a kvartérní amoniové sloučeniny $x chemie $7 D000644
- 650 _2
- $a fluorescenční spektrometrie $7 D013050
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kwiatek, Joanna M $u EMBL Australia Node in Single Molecule Science, School of Medical Sciences, Sydney, New South Wales, Australia; ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia.
- 700 1_
- $a Ma, Yuanqing $u EMBL Australia Node in Single Molecule Science, School of Medical Sciences, Sydney, New South Wales, Australia; ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia.
- 700 1_
- $a Benda, Aleš $u Imaging Methods Core Facility at BIOCEV, Faculty of Sciences, Charles University, Vestec, Czech Republic.
- 700 1_
- $a Gaus, Katharina $u EMBL Australia Node in Single Molecule Science, School of Medical Sciences, Sydney, New South Wales, Australia; ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia.
- 773 0_
- $w MED00000774 $t Biophysical journal $x 1542-0086 $g Roč. 114, č. 12 (2018), s. 2855-2864
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29925022 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20191007 $b ABA008
- 991 __
- $a 20191011085617 $b ABA008
- 999 __
- $a ok $b bmc $g 1451837 $s 1073727
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 114 $c 12 $d 2855-2864 $e 20180619 $i 1542-0086 $m Biophysical journal $n Biophys J $x MED00000774
- LZP __
- $a Pubmed-20191007
