• Je něco špatně v tomto záznamu ?

Various AKIP1 expression levels affect its subcellular localization but have no effect on NF-kappaB activation

A. Keprová, L. Kořínková, I. Křížová, R. Hadravová, F. Kaufman, I. Pichová, T. Ruml, M. Rumlová

. 2019 ; 68 (3) : 431-443. [pub] 20190322

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19043779

A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteins involved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation. While highly expressed AKIP1 accumulated in electron-dense aggregates both in the nucleus and cytosol, low expression of AKIP1 resulted in its localization within the nucleus as a free, non-aggregated protein. Even though AKIP1 was shown to interact with p65 subunit of NF-kappaB and activate this transcription factor, we did not observe any effect on NF-kappaB activation regardless of various AKIP1 expression level.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc19043779
003      
CZ-PrNML
005      
20200122082457.0
007      
ta
008      
200107s2019 xr ad f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933961 $2 doi
035    __
$a (PubMed)30904007
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Keprová, Alena. $7 xx0243695 $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic; Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic
245    10
$a Various AKIP1 expression levels affect its subcellular localization but have no effect on NF-kappaB activation / $c A. Keprová, L. Kořínková, I. Křížová, R. Hadravová, F. Kaufman, I. Pichová, T. Ruml, M. Rumlová
504    __
$a Literatura
520    9_
$a A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteins involved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation. While highly expressed AKIP1 accumulated in electron-dense aggregates both in the nucleus and cytosol, low expression of AKIP1 resulted in its localization within the nucleus as a free, non-aggregated protein. Even though AKIP1 was shown to interact with p65 subunit of NF-kappaB and activate this transcription factor, we did not observe any effect on NF-kappaB activation regardless of various AKIP1 expression level.
650    _2
$a adaptorové proteiny signální transdukční $x biosyntéza $x genetika $7 D048868
650    _2
$a buněčné jádro $x chemie $x metabolismus $7 D002467
650    _2
$a cytosol $x chemie $x metabolismus $7 D003600
650    _2
$a regulace genové exprese $7 D005786
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a lidé $7 D006801
650    _2
$a mitochondrie $x chemie $x metabolismus $7 D008928
650    _2
$a NF-kappa B $x analýza $x metabolismus $7 D016328
650    _2
$a jaderné proteiny $x biosyntéza $x genetika $7 D009687
650    _2
$a subcelulární frakce $x chemie $x metabolismus $7 D013347
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kořínková, Lucia. $7 xx0243700 $u Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic
700    1_
$a Křížová, Ivana. $7 xx0243686 $u Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic
700    1_
$a Hadravová, Romana $7 xx0274515 $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic
700    1_
$a Kaufman, Filip. $7 xx0243697 $u Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic
700    1_
$a Pichová, Iva $7 xx0101840 $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic
700    1_
$a Ruml, Tomáš, $d 1954- $7 nlk20030128618 $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czech Republic
700    1_
$a Rumlová, Michaela, $d 1968- $7 ola20040226003 $u Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 68, č. 3 (2019), s. 431-443
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30904007 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y p $z 0
990    __
$a 20200107 $b ABA008
991    __
$a 20200122082834 $b ABA008
999    __
$a ok $b bmc $g 1485091 $s 1082446
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 68 $c 3 $d 431-443 $e 20190322 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20200107

Najít záznam

Citační ukazatele

Možnosti archivace