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Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

N. Jaime-Pérez, D. Kaftan, D. Bína, SNH. Bokhari, S. Shreedhar, H. Küpper,

. 2019 ; 1860 (8) : 640-650. [pub] 20190619

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19044742

Magnesium (Mg2+) is the ubiquitous metal ion present in chlorophyll and bacteriochlorophyll (BChl), involved in photosystems in photosynthetic organisms. In the present study we investigated targets of toxic copper binding to the photosynthetic apparatus of the anoxygenic purple bacterium Rhodospirillum rubrum. This was done by a combination of in vivo measurements of flash photolysis and fast fluorescence kinetics combined with the analysis of metal binding to pigments and pigment-protein complexes isolated from Cu-stressed cells by HPLC-ICPMS (ICP-sfMS). This work concludes that R. rubrum is highly sensitive to Cu2+, with a strong inhibition of the photosynthetic reaction centres (RCs) already at 2 μM Cu2+. The inhibition of growth and of RC activity was related to the formation of Cu-containing BChl degradation products that occurred much more in the RC than in LH1. These results suggest that the shift of metal centres in BChl from Mg2+ to Cu2+ can occur in vivo in the RCs of R. rubrum under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of the function of these RCs.

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$a Magnesium (Mg2+) is the ubiquitous metal ion present in chlorophyll and bacteriochlorophyll (BChl), involved in photosystems in photosynthetic organisms. In the present study we investigated targets of toxic copper binding to the photosynthetic apparatus of the anoxygenic purple bacterium Rhodospirillum rubrum. This was done by a combination of in vivo measurements of flash photolysis and fast fluorescence kinetics combined with the analysis of metal binding to pigments and pigment-protein complexes isolated from Cu-stressed cells by HPLC-ICPMS (ICP-sfMS). This work concludes that R. rubrum is highly sensitive to Cu2+, with a strong inhibition of the photosynthetic reaction centres (RCs) already at 2 μM Cu2+. The inhibition of growth and of RC activity was related to the formation of Cu-containing BChl degradation products that occurred much more in the RC than in LH1. These results suggest that the shift of metal centres in BChl from Mg2+ to Cu2+ can occur in vivo in the RCs of R. rubrum under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of the function of these RCs.
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$a Kaftan, David $u University of South Bohemia, Faculty of Science, Institute of Chemistry, Branišovská 1760, 370 05 České Budějovice, Czech Republic; Centre Algatech, Institute of Microbiology CAS, 37981 Třeboň, Czech Republic.
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$a Bína, David $u University of South Bohemia, Faculty of Science, Institute of Chemistry, Branišovská 1760, 370 05 České Budějovice, Czech Republic; Biology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Photosynthesis, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic.
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$a Küpper, Hendrik $u Biology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Plant Biophysics & Biochemistry, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic; University of South Bohemia, Faculty of Science, Department of Experimental Plant Biology, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic. Electronic address: Hendrik.kuepper@umbr.cas.cz.
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