Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Systematic investigation of sequence requirements for DNA i-motif formation

P. Školáková, D. Renčiuk, J. Palacký, D. Krafčík, Z. Dvořáková, I. Kejnovská, K. Bednářová, M. Vorlíčková,

. 2019 ; 47 (5) : 2177-2189. [pub] 20190318

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045017

The formation of intercalated motifs (iMs) - secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is reflected by typical changes in CD and UV absorption spectra. By means of spectroscopic methods, electrophoresis, chemical modifications and other procedures, we characterized iM formation and stability in sequences with different cytosine block lengths interrupted by various numbers and types of nucleotides. Particular attention was paid to the formation of iMs at pH conditions close to neutral. We identified the optimal conditions and minimal requirements for iM formation in DNA sequences, and addressed gaps and inaccurate data interpretations in existing studies to specify principles of iM formation and modes of their folding.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19045017
003      
CZ-PrNML
005      
20200113081612.0
007      
ta
008      
200109s2019 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/nar/gkz046 $2 doi
035    __
$a (PubMed)30715498
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Školáková, Petra $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
245    10
$a Systematic investigation of sequence requirements for DNA i-motif formation / $c P. Školáková, D. Renčiuk, J. Palacký, D. Krafčík, Z. Dvořáková, I. Kejnovská, K. Bednářová, M. Vorlíčková,
520    9_
$a The formation of intercalated motifs (iMs) - secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is reflected by typical changes in CD and UV absorption spectra. By means of spectroscopic methods, electrophoresis, chemical modifications and other procedures, we characterized iM formation and stability in sequences with different cytosine block lengths interrupted by various numbers and types of nucleotides. Particular attention was paid to the formation of iMs at pH conditions close to neutral. We identified the optimal conditions and minimal requirements for iM formation in DNA sequences, and addressed gaps and inaccurate data interpretations in existing studies to specify principles of iM formation and modes of their folding.
650    _2
$a párování bází $7 D020029
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a cytosin $x chemie $x metabolismus $7 D003596
650    _2
$a DNA $x chemie $x metabolismus $7 D004247
650    _2
$a koncentrace vodíkových iontů $7 D006863
650    _2
$a kinetika $7 D007700
650    12
$a konformace nukleové kyseliny $7 D009690
650    12
$a nukleotidové motivy $7 D059372
650    _2
$a termodynamika $7 D013816
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Renčiuk, Daniel $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
700    1_
$a Palacký, Jan $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
700    1_
$a Krafčík, Daniel $u CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 735/5, 625 00 Brno, Czech Republic.
700    1_
$a Dvořáková, Zuzana $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
700    1_
$a Kejnovská, Iva $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
700    1_
$a Bednářová, Klára $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
700    1_
$a Vorlíčková, Michaela $u Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
773    0_
$w MED00003554 $t Nucleic acids research $x 1362-4962 $g Roč. 47, č. 5 (2019), s. 2177-2189
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30715498 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200109 $b ABA008
991    __
$a 20200113081944 $b ABA008
999    __
$a ok $b bmc $g 1483286 $s 1083690
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 47 $c 5 $d 2177-2189 $e 20190318 $i 1362-4962 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a Pubmed-20200109

Najít záznam

Citační ukazatele

Možnosti archivace