Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Correlation of Autophagosome Formation with Degradation and Endocytosis Arabidopsis Regulator of G-Protein Signaling (RGS1) through ATG8a

Y. Jiao, M. Srba, J. Wang, W. Chen,

. 2019 ; 20 (17) : . [pub] 20190827

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc20005975

Grant support
R01 GM065989 NIGMS NIH HHS - United States
31170250 National Natural Science Foundation of China
31570256 National Natural Science Foundation of China

Damaged or unwanted cellular proteins are degraded by either autophagy or the ubiquitin/proteasome pathway. In Arabidopsis thaliana, sensing of D-glucose is achieved by the heterotrimeric G protein complex and regulator of G-protein signaling 1 (AtRGS1). Here, we showed that starvation increases proteasome-independent AtRGS1 degradation, and it is correlated with increased autophagic flux. RGS1 promoted the production of autophagosomes and autophagic flux; RGS1-yellow fluorescent protein (YFP) was surrounded by vacuolar dye FM4-64 (red fluorescence). RGS1 and autophagosomes co-localized in the root cells of Arabidopsis and BY-2 cells. We demonstrated that the autophagosome marker ATG8a interacts with AtRGS1 and its shorter form with truncation of the seven transmembrane and RGS1 domains in planta. Altogether, our data indicated the correlation of autophagosome formation with degradation and endocytosis of AtRGS1 through ATG8a.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20005975
003      
CZ-PrNML
005      
20200518132230.0
007      
ta
008      
200511s2019 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms20174190 $2 doi
035    __
$a (PubMed)31461856
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Jiao, Yue $u MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. College of Biophotonics, South China Normal University, Guangzhou 510631, China.
245    10
$a Correlation of Autophagosome Formation with Degradation and Endocytosis Arabidopsis Regulator of G-Protein Signaling (RGS1) through ATG8a / $c Y. Jiao, M. Srba, J. Wang, W. Chen,
520    9_
$a Damaged or unwanted cellular proteins are degraded by either autophagy or the ubiquitin/proteasome pathway. In Arabidopsis thaliana, sensing of D-glucose is achieved by the heterotrimeric G protein complex and regulator of G-protein signaling 1 (AtRGS1). Here, we showed that starvation increases proteasome-independent AtRGS1 degradation, and it is correlated with increased autophagic flux. RGS1 promoted the production of autophagosomes and autophagic flux; RGS1-yellow fluorescent protein (YFP) was surrounded by vacuolar dye FM4-64 (red fluorescence). RGS1 and autophagosomes co-localized in the root cells of Arabidopsis and BY-2 cells. We demonstrated that the autophagosome marker ATG8a interacts with AtRGS1 and its shorter form with truncation of the seven transmembrane and RGS1 domains in planta. Altogether, our data indicated the correlation of autophagosome formation with degradation and endocytosis of AtRGS1 through ATG8a.
650    _2
$a Arabidopsis $7 D017360
650    _2
$a proteiny huseníčku $x genetika $x metabolismus $7 D029681
650    _2
$a autofagozomy $x metabolismus $7 D000071182
650    _2
$a autofagie $7 D001343
650    _2
$a rodina proteinů Atg8 $x genetika $x metabolismus $7 D000071190
650    _2
$a endocytóza $7 D004705
650    _2
$a proteolýza $7 D059748
650    _2
$a proteiny RGS $x genetika $x metabolismus $7 D020710
650    _2
$a signální transdukce $7 D015398
655    _2
$a časopisecké články $7 D016428
700    1_
$a Srba, Miroslav $u Department of Experimental Plant Biology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
700    1_
$a Wang, Jingchun $u MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. College of Biophotonics, South China Normal University, Guangzhou 510631, China.
700    1_
$a Chen, Wenli $u MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. chenwl@scnu.edu.cn. College of Biophotonics, South China Normal University, Guangzhou 510631, China. chenwl@scnu.edu.cn.
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 20, č. 17 (2019)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31461856 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20200518132230 $b ABA008
999    __
$a ok $b bmc $g 1524833 $s 1096031
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 20 $c 17 $e 20190827 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a R01 GM065989 $p NIGMS NIH HHS $2 United States
GRA    __
$a 31170250 $p National Natural Science Foundation of China
GRA    __
$a 31570256 $p National Natural Science Foundation of China
LZP    __
$a Pubmed-20200511

Find record

Citation metrics

Logged in users only

Archiving options