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The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

O. Porubiaková, N. Bohálová, A. Inga, N. Vadovičová, J. Coufal, M. Fojta, V. Brázda,

. 2019 ; 21 (1) : . [pub] 20191224

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc20023312

Grant support
18-15548S Grantová Agentura České Republiky
CZ.02.1.01/0.0/0.0/15 003/0000477 European Regional Development Fund

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53α isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53α isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53α isoforms.

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$a Brázda, Václav $u Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 61200 Brno, Czech Republic. Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 61265 Brno, Czech Republic.
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