Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Europium (III) as a Circularly Polarized Luminescence Probe of DNA Structure

T. Wu, P. Bouř, V. Andrushchenko,

. 2019 ; 9 (1) : 1068. [pub] 20190131

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20025954

We report as a proof-of-concept the first application of circularly polarized luminescence (CPL) measured with a Raman optical activity (ROA) spectrometer to differentiate several DNA structures without need of sensitizing complexes. The ROA/CPL approach provides sufficiently high CPL intensity to use hydrated Eu3+ ions, thus avoiding DNA structural changes associated with binding of sensitizers and overcoming the sensitizer quenching issue. We showed that deoxyguanosine monophosphate (dGMP), single- and double-stranded DNA provide different CPL spectra, which could be used for their discrimination. Our results demonstrate that ROA/CPL method is a promising approach to measure CPL spectra of complex biomolecules when the use of sensitizers is not possible. The method can be extended to other biomolecules, such as proteins, lipids, sugars, etc.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025954
003      
CZ-PrNML
005      
20201222154225.0
007      
ta
008      
201125s2019 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-018-37680-7 $2 doi
035    __
$a (PubMed)30705327
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Wu, Tao $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo náměstí 2, 16610, Prague 6, Czech Republic.
245    10
$a Europium (III) as a Circularly Polarized Luminescence Probe of DNA Structure / $c T. Wu, P. Bouř, V. Andrushchenko,
520    9_
$a We report as a proof-of-concept the first application of circularly polarized luminescence (CPL) measured with a Raman optical activity (ROA) spectrometer to differentiate several DNA structures without need of sensitizing complexes. The ROA/CPL approach provides sufficiently high CPL intensity to use hydrated Eu3+ ions, thus avoiding DNA structural changes associated with binding of sensitizers and overcoming the sensitizer quenching issue. We showed that deoxyguanosine monophosphate (dGMP), single- and double-stranded DNA provide different CPL spectra, which could be used for their discrimination. Our results demonstrate that ROA/CPL method is a promising approach to measure CPL spectra of complex biomolecules when the use of sensitizers is not possible. The method can be extended to other biomolecules, such as proteins, lipids, sugars, etc.
650    _2
$a DNA $x chemie $7 D004247
650    _2
$a deoxyguaninnukleotidy $x chemie $7 D003848
650    _2
$a europium $x chemie $7 D005063
650    _2
$a luminiscence $7 D049449
650    _2
$a Ramanova spektroskopie $x metody $7 D013059
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Bouř, Petr $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo náměstí 2, 16610, Prague 6, Czech Republic.
700    1_
$a Andrushchenko, Valery $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo náměstí 2, 16610, Prague 6, Czech Republic. andrushchenko@uochb.cas.cz.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 9, č. 1 (2019), s. 1068
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30705327 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201222154221 $b ABA008
999    __
$a ok $b bmc $g 1600099 $s 1116640
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 9 $c 1 $d 1068 $e 20190131 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20201125

Find record

Citation metrics

Archiving options