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Lipidomic characterization of exosomes isolated from human plasma using various mass spectrometry techniques
O. Peterka, R. Jirásko, M. Chocholoušková, L. Kuchař, D. Wolrab, R. Hájek, D. Vrána, O. Strouhal, B. Melichar, M. Holčapek,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Diglycerides blood isolation & purification metabolism MeSH
- Adult MeSH
- Exosomes chemistry metabolism MeSH
- Phosphatidylcholines blood isolation & purification metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Lipidomics methods MeSH
- Lysophosphatidylcholines blood isolation & purification metabolism MeSH
- Lipid Metabolism * MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Chromatography, Supercritical Fluid methods MeSH
- Triglycerides blood isolation & purification metabolism MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Healthy Volunteers MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Ultrahigh-performance supercritical fluid chromatography - mass spectrometry (UHPSFC/MS), ultrahigh-performance liquid chromatography - mass spectrometry (UHPLC/MS), and matrix-assisted laser desorption/ionization (MALDI) - MS techniques were used for the lipidomic characterization of exosomes isolated from human plasma. The high-throughput methods UHPSFC/MS and UHPLC/MS using a silica-based column containing sub-2 μm particles enabled the lipid class separation and the quantitation based on exogenous class internal standards in <7 minute run time. MALDI provided the complementary information on anionic lipid classes, such as sulfatides. The nontargeted analysis of 12 healthy volunteers was performed, and absolute molar concentration of 244 lipids in exosomes and 191 lipids in plasma belonging to 10 lipid classes were quantified. The statistical evaluation of data included principal component analysis, orthogonal partial least square discriminant analysis, S-plots, p-values, T-values, fold changes, false discovery rate, box plots, and correlation plots, which resulted in the information on lipid changes in exosomes in comparison to plasma. The major changes were detected in the composition of triacylglycerols, diacylglycerols, phosphatidylcholines, and lysophosphatidylcholines, whereby sphingomyelins, phosphatidylinositols, and sulfatides showed rather similar profiles in both biological matrices.
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