Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Oxidative Stress and Analysis of Selected SNPs of ACHE (rs 2571598), BCHE (rs 3495), CAT (rs 7943316), SIRT1 (rs 10823108), GSTP1 (rs 1695), and Gene GSTM1, GSTT1 in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan

LJ. Mbah Ntepe, R. Habib, N. Judith Laure, S. Raza, E. Nepovimova, K. Kuca, S. Batool, S. Muhammad Nurulain

. 2020 ; 21 (17) : . [pub] 20200903

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21012159

Grant support
VT2019-2021 UHK CEP Register
Internal project COMSATS University Islamabad
Internal project The World Academy of Sciences (TWAS)

The detrimental effects of organophosphates (OPs) on human health are thought to be of systemic, i.e., irreversible inhibition of acetylcholinesterase (AChE) at nerve synapses. However, several studies have shown that AChE inhibition alone cannot explain all the toxicological manifestations in prolonged exposure to OPs. The present study aimed to assess the status of antioxidants malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) (reduced), catalase, and ferric reducing antioxidant power (FRAP) in chronic OP-exposed groups from Cameroon and Pakistan. Molecular analysis of genetic polymorphisms (SNPs) of glutathione transferases (GSTM1, GSTP1, GSTT1), catalase gene (CAT, rs7943316), sirtuin 1 gene (SIRT1, rs10823108), acetylcholinesterase gene (ACHE, rs2571598), and butyrylcholinesterase gene (BCHE, rs3495) were screened in the OP-exposed individuals to find the possible causative association with oxidative stress and toxicity. Cholinesterase and antioxidant activities were measured by colorimetric methods using a spectrophotometer. Salting-out method was employed for DNA extraction from blood followed by restriction fragment length polymorphism (RFLP) for molecular analysis. Cholinergic enzymes were significantly decreased in OP-exposed groups. Catalase and SOD were decreased and MDA and FRAP were increased in OP-exposed groups compared to unexposed groups in both groups. GSH was decreased only in Pakistani OPs-exposed group. Molecular analysis of ACHE, BCHE, Catalase, GSTP1, and GSTM1 SNPs revealed a tentative association with their phenotypic expression that is level of antioxidant and cholinergic enzymes. The study concludes that chronic OPs exposure induces oxidative stress which is associated with the related SNP polymorphism. The toxicogenetics of understudied SNPs were examined for the first time to our understanding. The findings may lead to a newer area of investigation on OPs induced health issues and toxicogenetics.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21012159
003      
CZ-PrNML
005      
20210507102705.0
007      
ta
008      
210420s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms21176432 $2 doi
035    __
$a (PubMed)32899431
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Mbah Ntepe, Leonel Javeres $u Department of Biosciences, COMSATS University Islamabad, Chak Shahzad, Islamabad 45550, Pakistan
245    10
$a Oxidative Stress and Analysis of Selected SNPs of ACHE (rs 2571598), BCHE (rs 3495), CAT (rs 7943316), SIRT1 (rs 10823108), GSTP1 (rs 1695), and Gene GSTM1, GSTT1 in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan / $c LJ. Mbah Ntepe, R. Habib, N. Judith Laure, S. Raza, E. Nepovimova, K. Kuca, S. Batool, S. Muhammad Nurulain
520    9_
$a The detrimental effects of organophosphates (OPs) on human health are thought to be of systemic, i.e., irreversible inhibition of acetylcholinesterase (AChE) at nerve synapses. However, several studies have shown that AChE inhibition alone cannot explain all the toxicological manifestations in prolonged exposure to OPs. The present study aimed to assess the status of antioxidants malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) (reduced), catalase, and ferric reducing antioxidant power (FRAP) in chronic OP-exposed groups from Cameroon and Pakistan. Molecular analysis of genetic polymorphisms (SNPs) of glutathione transferases (GSTM1, GSTP1, GSTT1), catalase gene (CAT, rs7943316), sirtuin 1 gene (SIRT1, rs10823108), acetylcholinesterase gene (ACHE, rs2571598), and butyrylcholinesterase gene (BCHE, rs3495) were screened in the OP-exposed individuals to find the possible causative association with oxidative stress and toxicity. Cholinesterase and antioxidant activities were measured by colorimetric methods using a spectrophotometer. Salting-out method was employed for DNA extraction from blood followed by restriction fragment length polymorphism (RFLP) for molecular analysis. Cholinergic enzymes were significantly decreased in OP-exposed groups. Catalase and SOD were decreased and MDA and FRAP were increased in OP-exposed groups compared to unexposed groups in both groups. GSH was decreased only in Pakistani OPs-exposed group. Molecular analysis of ACHE, BCHE, Catalase, GSTP1, and GSTM1 SNPs revealed a tentative association with their phenotypic expression that is level of antioxidant and cholinergic enzymes. The study concludes that chronic OPs exposure induces oxidative stress which is associated with the related SNP polymorphism. The toxicogenetics of understudied SNPs were examined for the first time to our understanding. The findings may lead to a newer area of investigation on OPs induced health issues and toxicogenetics.
650    _2
$a acetylcholinesterasa $x genetika $7 D000110
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a butyrylcholinesterasa $x genetika $7 D002091
650    _2
$a katalasa $x genetika $7 D002374
650    _2
$a vystavení vlivu životního prostředí $x škodlivé účinky $x analýza $7 D004781
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a GPI-vázané proteiny $x genetika $7 D058851
650    12
$a interakce genů a prostředí $7 D059647
650    _2
$a glutathion $7 D005978
650    _2
$a glutathion-S-transferasa fí $x genetika $7 D051549
650    _2
$a glutathiontransferasa $x genetika $7 D005982
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a malondialdehyd $7 D008315
650    _2
$a lidé středního věku $7 D008875
650    _2
$a organofosforové sloučeniny $x škodlivé účinky $7 D009943
650    _2
$a oxidační stres $x genetika $7 D018384
650    12
$a jednonukleotidový polymorfismus $7 D020641
650    _2
$a sirtuin 1 $x genetika $7 D056564
650    _2
$a mladý dospělý $7 D055815
651    _2
$a Kamerun $7 D002163
651    _2
$a Pákistán $7 D010154
655    _2
$a časopisecké články $7 D016428
700    1_
$a Habib, Rabia $u Department of Biosciences, COMSATS University Islamabad, Chak Shahzad, Islamabad 45550, Pakistan
700    1_
$a Judith Laure, Ngondi $u Department of Biochemistry, Yaoundé I University, Yaoundé 8024, Cameroon
700    1_
$a Raza, Saqlain $u Department of Mathematics, COMSATS University Islamabad, Chak Shahzad, Islamabad 45550, Pakistan
700    1_
$a Nepovimova, Eugenie $u Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic
700    1_
$a Kuca, Kamil $u Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic
700    1_
$a Batool, Sajida $u Department of Biosciences, COMSATS University Islamabad, Chak Shahzad, Islamabad 45550, Pakistan
700    1_
$a Muhammad Nurulain, Syed $u Department of Biosciences, COMSATS University Islamabad, Chak Shahzad, Islamabad 45550, Pakistan
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 21, č. 17 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32899431 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210507102704 $b ABA008
999    __
$a ok $b bmc $g 1650518 $s 1132538
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 21 $c 17 $e 20200903 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a VT2019-2021 $p UHK
GRA    __
$a Internal project $p COMSATS University Islamabad
GRA    __
$a Internal project $p The World Academy of Sciences (TWAS)
LZP    __
$a Pubmed-20210420

Find record

Citation metrics

Archiving options