Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST457 (14 isolates), ST38 (5), ST10 (2), ST117 (2) or ST93 (4) were detected. Isolates from three farms, which purchased chicken from a Paraguayan hatchery showed higher prevalence of mcr-5.1 and blaCTX-M-8 compared to the other nine farms, which purchased chickens from a Brazilian hatchery. Moreover, none of the K. pneumoniae isolates were linked to the Paraguayan hatchery. ESBL/AmpC and mcr-5-carrying multi-drug resistant (MDR) plasmids were characterized, and complete sequences were obtained for eight plasmids. The study shed light on Paraguayan poultry farms as a reservoir of antibiotic resistance commonly conferred via MDR plasmids and showed linkage between resistance and origin of the chickens at the hatcheries level.

Biomedical Centre Charles University 32300 Pilsen Czech Republic

CEITEC VETUNI University of Veterinary Sciences Brno 61242 Brno Czech Republic

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary Sciences Brno 61242 Brno Czech Republic

Department of Microbiology University Hospital of Larissa 41110 Larissa Greece

Faculty of Medicine in Pilsen Charles University 30605 Pilsen Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc21017943

003      

CZ-PrNML

005      

20210729104058.0

007      

ta

008      

210726s2021 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/microorganisms9040866 $2 doi

035    __

$a (PubMed)33920558

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Nesporova, Kristina $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic $u Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic

245    10

$a Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries / $c K. Nesporova, A. Valcek, C. Papagiannitsis, I. Kutilova, I. Jamborova, L. Davidova-Gerzova, I. Bitar, J. Hrabak, I. Literak, M. Dolejska

520    9_

$a Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST457 (14 isolates), ST38 (5), ST10 (2), ST117 (2) or ST93 (4) were detected. Isolates from three farms, which purchased chicken from a Paraguayan hatchery showed higher prevalence of mcr-5.1 and blaCTX-M-8 compared to the other nine farms, which purchased chickens from a Brazilian hatchery. Moreover, none of the K. pneumoniae isolates were linked to the Paraguayan hatchery. ESBL/AmpC and mcr-5-carrying multi-drug resistant (MDR) plasmids were characterized, and complete sequences were obtained for eight plasmids. The study shed light on Paraguayan poultry farms as a reservoir of antibiotic resistance commonly conferred via MDR plasmids and showed linkage between resistance and origin of the chickens at the hatcheries level.

655    _2

$a časopisecké články $7 D016428

700    1_

$a Valcek, Adam $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic $u Biomedical Centre, Charles University, 32300 Pilsen, Czech Republic

700    1_

$a Papagiannitsis, Costas $u Department of Microbiology, University Hospital of Larissa, 41110 Larissa, Greece

700    1_

$a Kutilova, Iva $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic

700    1_

$a Jamborova, Ivana $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic

700    1_

$a Davidova-Gerzova, Lenka $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic

700    1_

$a Bitar, Ibrahim $u Biomedical Centre, Charles University, 32300 Pilsen, Czech Republic $u Faculty of Medicine in Pilsen, Charles University, 30605 Pilsen, Czech Republic

700    1_

$a Hrabak, Jaroslav $u Biomedical Centre, Charles University, 32300 Pilsen, Czech Republic $u Faculty of Medicine in Pilsen, Charles University, 30605 Pilsen, Czech Republic

700    1_

$a Literak, Ivan $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic $u Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic

700    1_

$a Dolejska, Monika $u CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic $u Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic $u Biomedical Centre, Charles University, 32300 Pilsen, Czech Republic

773    0_

$w MED00198767 $t Microorganisms $x 2076-2607 $g Roč. 9, č. 4 (2021)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/33920558 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y - $z 0

990    __

$a 20210726 $b ABA008

991    __

$a 20210729104057 $b ABA008

999    __

$a ind $b bmc $g 1676474 $s 1138385

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2021 $b 9 $c 4 $e 20210417 $i 2076-2607 $m Microorganisms $n Microorganisms $x MED00198767

GRA    __

$a CEITEC/Literak/ITA2021 2021ITA31 $p Internal Grant Agency of University of Veterinary and Pharmaceutical Sciences Brno

LZP    __

$a Pubmed-20210726