Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Defective viral genomes from chikungunya virus are broad-spectrum antivirals and prevent virus dissemination in mosquitoes

LI. Levi, VV. Rezelj, A. Henrion-Lacritick, D. Erazo, J. Boussier, T. Vallet, V. Bernhauerová, Y. Suzuki, L. Carrau, J. Weger-Lucarelli, MC. Saleh, M. Vignuzzi

. 2021 ; 17 (2) : e1009110. [pub] 20210208

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.

Perzistentní odkaz   https://www.medvik.cz/link/bmc21019272

Defective viral genomes (DVGs) are truncated and/or rearranged viral genomes produced during virus replication. Described in many RNA virus families, some of them have interfering activity on their parental virus and/or strong immunostimulatory potential, and are being considered in antiviral approaches. Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes spp. that infected millions of humans in the last 15 years. Here, we describe the DVGs arising during CHIKV infection in vitro in mammalian and mosquito cells, and in vivo in experimentally infected Aedes aegypti mosquitoes. We combined experimental and computational approaches to select DVG candidates most likely to have inhibitory activity and showed that, indeed, they strongly interfere with CHIKV replication both in mammalian and mosquito cells. We further demonstrated that some DVGs present broad-spectrum activity, inhibiting several CHIKV strains and other alphaviruses. Finally, we showed that pre-treating Aedes aegypti with DVGs prevented viral dissemination in vivo.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21019272
003      
CZ-PrNML
005      
20210830100842.0
007      
ta
008      
210728s2021 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.ppat.1009110 $2 doi
035    __
$a (PubMed)33556143
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Levi, Laura I $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France $u École doctorale BioSPC, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
245    10
$a Defective viral genomes from chikungunya virus are broad-spectrum antivirals and prevent virus dissemination in mosquitoes / $c LI. Levi, VV. Rezelj, A. Henrion-Lacritick, D. Erazo, J. Boussier, T. Vallet, V. Bernhauerová, Y. Suzuki, L. Carrau, J. Weger-Lucarelli, MC. Saleh, M. Vignuzzi
520    9_
$a Defective viral genomes (DVGs) are truncated and/or rearranged viral genomes produced during virus replication. Described in many RNA virus families, some of them have interfering activity on their parental virus and/or strong immunostimulatory potential, and are being considered in antiviral approaches. Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes spp. that infected millions of humans in the last 15 years. Here, we describe the DVGs arising during CHIKV infection in vitro in mammalian and mosquito cells, and in vivo in experimentally infected Aedes aegypti mosquitoes. We combined experimental and computational approaches to select DVG candidates most likely to have inhibitory activity and showed that, indeed, they strongly interfere with CHIKV replication both in mammalian and mosquito cells. We further demonstrated that some DVGs present broad-spectrum activity, inhibiting several CHIKV strains and other alphaviruses. Finally, we showed that pre-treating Aedes aegypti with DVGs prevented viral dissemination in vivo.
650    _2
$a Aedes $x virologie $7 D000330
650    _2
$a zvířata $7 D000818
650    _2
$a antivirové látky $x farmakologie $7 D000998
650    _2
$a horečka chikungunya $x imunologie $x přenos $x virologie $7 D065632
650    _2
$a virus chikungunya $x genetika $x růst a vývoj $x izolace a purifikace $7 D002646
650    _2
$a defektní viry $x genetika $7 D003673
650    12
$a genom virový $7 D016679
650    _2
$a lidé $7 D006801
650    _2
$a komáří přenašeči $x virologie $7 D000072138
650    12
$a replikace viru $7 D014779
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
655    _2
$a Research Support, U.S. Gov't, Non-P.H.S. $7 D013486
700    1_
$a Rezelj, Veronica V $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France
700    1_
$a Henrion-Lacritick, Annabelle $u Institut Pasteur, Viruses and RNAi Unit, CNRS UMR 3569, Paris, France
700    1_
$a Erazo, Diana $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France
700    1_
$a Boussier, J $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France $u École doctorale Frontières du vivant, Université Paris Diderot, Paris, France
700    1_
$a Vallet, Thomas $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France
700    1_
$a Bernhauerová, Veronika $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France $u Department of Biophysics and Physical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic
700    1_
$a Suzuki, Yasutsugu $u Institut Pasteur, Viruses and RNAi Unit, CNRS UMR 3569, Paris, France
700    1_
$a Carrau, Lucia $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France $u École doctorale BioSPC, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
700    1_
$a Weger-Lucarelli, James $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France $u Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, Virginia, United States of America
700    1_
$a Saleh, Maria-Carla $u Institut Pasteur, Viruses and RNAi Unit, CNRS UMR 3569, Paris, France
700    1_
$a Vignuzzi, Marco $u Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Paris, France
773    0_
$w MED00008922 $t PLoS pathogens $x 1553-7374 $g Roč. 17, č. 2 (2021), s. e1009110
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33556143 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830100842 $b ABA008
999    __
$a ok $b bmc $g 1690163 $s 1139718
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 17 $c 2 $d e1009110 $e 20210208 $i 1553-7374 $m PLOS pathogens $n PLoS Pathog $x MED00008922
LZP    __
$a Pubmed-20210728

Najít záznam

Citační ukazatele

Možnosti archivace