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An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast
J. Sumbal, T. Gudjonsson, GA. Traustadottir, Z. Koledova
Language English Country United States
Document type Journal Article
- MeSH
- Stromal Cells MeSH
- Epithelial Cells MeSH
- Fibroblasts MeSH
- Humans MeSH
- Mammary Glands, Human * MeSH
- Mammary Glands, Animal MeSH
- Breast Neoplasms * MeSH
- Paracrine Communication MeSH
- Breast MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Epithelial-stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
Department of Laboratory Hematology Landspitali University Hospital Reykjavik Iceland
References provided by Crossref.org
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- $a Epithelial-stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.
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