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Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity
HIS. Thomas, YS. Chen, CH. Hung, DB. Sreerangaraja Urs, TL. Liao, YC. Lai, K. Komrskova, P. Postlerová, YF. Lin, SH. Kao
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Hindawi Publishing Open Access od 2008-01-01
PubMed Central od 2008
Europe PubMed Central od 2008
ProQuest Central od 2014-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2009-01-01
Medline Complete (EBSCOhost) od 2011-01-01
Health & Medicine (ProQuest) od 2014-01-01
Odkazy
PubMed
34527175
DOI
10.1155/2021/5561395
Knihovny.cz E-zdroje
- MeSH
- DNA-glykosylasy genetika metabolismus MeSH
- frekvence genu MeSH
- lidé MeSH
- membránový potenciál mitochondrií účinky léků MeSH
- mitochondriální DNA genetika metabolismus MeSH
- mitochondrie genetika metabolismus MeSH
- motilita spermií fyziologie MeSH
- mužská infertilita genetika patologie MeSH
- oxidační stres účinky léků MeSH
- peroxid vodíku farmakologie MeSH
- polymorfismus genetický MeSH
- spermie metabolismus fyziologie MeSH
- superoxiddismutasa genetika metabolismus MeSH
- uncoupling protein 2 genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Sperm motility is one of the major determinants of male fertility. Since sperm need a great deal of energy to support their fast movement by active metabolism, they are thus extremely vulnerable to oxidative damage by the reactive oxygen species (ROS) and other free radicals generated as byproducts in the electron transport chain. The present study is aimed at understanding the impact of a mitochondrial oxidizing/reducing microenvironment in the etiopathology of male infertility. We detected the mitochondrial DNA (mtDNA) 4,977 bp deletion in human sperm. We examined the gene mutation of ATP synthase 6 (ATPase6 m.T8993G) in ATP generation, the gene polymorphisms of uncoupling protein 2 (UCP2, G-866A) in the uncoupling of oxidative phosphorylation, the role of genes such as manganese superoxide dismutase (MnSOD, C47T) and catalase (CAT, C-262T) in the scavenging system in neutralizing reactive oxygen species, and the role of human 8-oxoguanine DNA glycosylase (hOGG1, C1245G) in 8-hydroxy-2'-deoxyguanosine (8-OHdG) repair. We found that the sperm with higher motility were found to have a higher mitochondrial membrane potential and mitochondrial bioenergetics. The genotype frequencies of UCP2 G-866A, MnSOD C47T, and CAT C-262T were found to be significantly different among the fertile subjects, the infertile subjects with more than 50% motility, and the infertile subjects with less than 50% motility. A higher prevalence of the mtDNA 4,977 bp deletion was found in the subjects with impaired sperm motility and fertility. Furthermore, we found that there were significant differences between the occurrences of the mtDNA 4,977 bp deletion and MnSOD (C47T) and hOGG1 (C1245G). In conclusion, the maintenance of the mitochondrial redox microenvironment and genome integrity is an important issue in sperm motility and fertility.
Center for Reproductive Medicine and Sciences Taipei Medical University Hospital Taipei Taiwan
Department of Gynecology and Obstetrics Taipei City Hospital Ren Ai Branch Taipei Taiwan
Department of Urology College of Medicine Taipei Medical University Taipei Taiwan
Department of Urology School of Medicine Fu Jen Catholic University New Taipei Taiwan
Department of Zoology Faculty of Science Charles University Prague Czech Republic
Division of Urology Department of Surgery Shin Kong Wu Ho Su Memorial Hospital Taipei Taiwan
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- $a Sperm motility is one of the major determinants of male fertility. Since sperm need a great deal of energy to support their fast movement by active metabolism, they are thus extremely vulnerable to oxidative damage by the reactive oxygen species (ROS) and other free radicals generated as byproducts in the electron transport chain. The present study is aimed at understanding the impact of a mitochondrial oxidizing/reducing microenvironment in the etiopathology of male infertility. We detected the mitochondrial DNA (mtDNA) 4,977 bp deletion in human sperm. We examined the gene mutation of ATP synthase 6 (ATPase6 m.T8993G) in ATP generation, the gene polymorphisms of uncoupling protein 2 (UCP2, G-866A) in the uncoupling of oxidative phosphorylation, the role of genes such as manganese superoxide dismutase (MnSOD, C47T) and catalase (CAT, C-262T) in the scavenging system in neutralizing reactive oxygen species, and the role of human 8-oxoguanine DNA glycosylase (hOGG1, C1245G) in 8-hydroxy-2'-deoxyguanosine (8-OHdG) repair. We found that the sperm with higher motility were found to have a higher mitochondrial membrane potential and mitochondrial bioenergetics. The genotype frequencies of UCP2 G-866A, MnSOD C47T, and CAT C-262T were found to be significantly different among the fertile subjects, the infertile subjects with more than 50% motility, and the infertile subjects with less than 50% motility. A higher prevalence of the mtDNA 4,977 bp deletion was found in the subjects with impaired sperm motility and fertility. Furthermore, we found that there were significant differences between the occurrences of the mtDNA 4,977 bp deletion and MnSOD (C47T) and hOGG1 (C1245G). In conclusion, the maintenance of the mitochondrial redox microenvironment and genome integrity is an important issue in sperm motility and fertility.
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