• Je něco špatně v tomto záznamu ?

A productive immunocompetent mouse model of cryptosporidiosis with long oocyst shedding duration for immunological studies

X. He, W. Huang, L. Sun, T. Hou, Z. Wan, N. Li, Y. Guo, M. Kváč, L. Xiao, Y. Feng

. 2022 ; 84 (5) : 710-721. [pub] 20220219

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22018598

OBJECTIVES: Studies on the pathogenesis and immune responses of Cryptosporidium infection and development of drugs and vaccines use mostly immunocompromised mouse models. In this study, we establish an immunocompetent mouse model of cryptosporidiosis with high intensity and long duration of infection. METHODS: We have obtained a Cryptosporidium tyzzeri isolate from laboratory mice, and infect adult C57BL/6 J mice experimentally with the isolate for determinations of infectivity, infection patterns, pathological changes, and transcriptomic responses. RESULTS: The isolate has an ID50 of 5.2 oocysts, with oocyst shedding lasting at high levels for >2 months. The oocyst shedding is boosted by immunosuppression of animals and suppressed by paromomycin treatment. The isolate induces strong inflammatory and acquired immune responses, but down-regulates the expression of α-defensins in epithelium. Comparative genomics analysis has revealed significant sequence differences from other isolates in subtelomeric genes. The down-regulation of the expression of α-defensins may be responsible for the high-intensity and long-lasting infection in this animal model. CONCLUSIONS: The immunocompetent mouse model of cryptosporidiosis developed has the advantages of high oocyst shedding intensity and long oocyst shedding duration. It provides an effective mechanism for the propagation of Cryptosporidium, evaluations of potential therapeutics, and studies of pathogen biology and immune responses.

000      
00000naa a2200000 a 4500
001      
bmc22018598
003      
CZ-PrNML
005      
20220804134904.0
007      
ta
008      
220720s2022 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.jinf.2022.02.019 $2 doi
035    __
$a (PubMed)35192895
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a He, Xi $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture. Guangzhou, Guangdong 510642, China
245    12
$a A productive immunocompetent mouse model of cryptosporidiosis with long oocyst shedding duration for immunological studies / $c X. He, W. Huang, L. Sun, T. Hou, Z. Wan, N. Li, Y. Guo, M. Kváč, L. Xiao, Y. Feng
520    9_
$a OBJECTIVES: Studies on the pathogenesis and immune responses of Cryptosporidium infection and development of drugs and vaccines use mostly immunocompromised mouse models. In this study, we establish an immunocompetent mouse model of cryptosporidiosis with high intensity and long duration of infection. METHODS: We have obtained a Cryptosporidium tyzzeri isolate from laboratory mice, and infect adult C57BL/6 J mice experimentally with the isolate for determinations of infectivity, infection patterns, pathological changes, and transcriptomic responses. RESULTS: The isolate has an ID50 of 5.2 oocysts, with oocyst shedding lasting at high levels for >2 months. The oocyst shedding is boosted by immunosuppression of animals and suppressed by paromomycin treatment. The isolate induces strong inflammatory and acquired immune responses, but down-regulates the expression of α-defensins in epithelium. Comparative genomics analysis has revealed significant sequence differences from other isolates in subtelomeric genes. The down-regulation of the expression of α-defensins may be responsible for the high-intensity and long-lasting infection in this animal model. CONCLUSIONS: The immunocompetent mouse model of cryptosporidiosis developed has the advantages of high oocyst shedding intensity and long oocyst shedding duration. It provides an effective mechanism for the propagation of Cryptosporidium, evaluations of potential therapeutics, and studies of pathogen biology and immune responses.
650    _2
$a zvířata $7 D000818
650    12
$a kryptosporidióza $x patologie $7 D003457
650    12
$a Cryptosporidium $x fyziologie $7 D003458
650    12
$a Cryptosporidium parvum $7 D016785
650    _2
$a modely nemocí na zvířatech $7 D004195
650    _2
$a feces $7 D005243
650    _2
$a lidé $7 D006801
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a oocysty $7 D033661
650    12
$a alfa-defensiny $7 D023084
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Huang, Wanyi $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
700    1_
$a Sun, Lianbei $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
700    1_
$a Hou, Tianyi $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
700    1_
$a Wan, Zhuowei $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
700    1_
$a Li, Na $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: nli@scau.edu.cn
700    1_
$a Guo, Yaqiong $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: guoyq@scau.edu.cn
700    1_
$a Kváč, Martin $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice 370 05, Czech Republic. Electronic address: kvac@paru.cas.cz
700    1_
$a Xiao, Lihua $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture. Guangzhou, Guangdong 510642, China. Electronic address: lxiao1961@gmail.com
700    1_
$a Feng, Yaoyu $u Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture. Guangzhou, Guangdong 510642, China. Electronic address: yyfeng@scau.edu.cn
773    0_
$w MED00002744 $t The Journal of infection $x 1532-2742 $g Roč. 84, č. 5 (2022), s. 710-721
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35192895 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804134858 $b ABA008
999    __
$a ok $b bmc $g 1822278 $s 1169841
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 84 $c 5 $d 710-721 $e 20220219 $i 1532-2742 $m The Journal of infection $n J Infect $x MED00002744
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Možnosti archivace