• Something wrong with this record ?

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

P. Švec, S. Králová, E. Staňková, P. Holochová, K. Sedlář, S. Koudelková, D. Krsek, J. Grzesiak, O. Šedo, P. Váczi, . Urvashi, V. Gupta, U. Sood, R. Lal, S. Korpole, I. Sedláček

. 2022 ; 72 (4) : . [pub] -

Language English Country Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22018981

Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA-DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22018981
003      
CZ-PrNML
005      
20220804135234.0
007      
ta
008      
220720s2022 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1099/ijsem.0.005309 $2 doi
035    __
$a (PubMed)35442878
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Švec, Pavel $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
245    10
$a Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica / $c P. Švec, S. Králová, E. Staňková, P. Holochová, K. Sedlář, S. Koudelková, D. Krsek, J. Grzesiak, O. Šedo, P. Váczi, . Urvashi, V. Gupta, U. Sood, R. Lal, S. Korpole, I. Sedláček
520    9_
$a Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA-DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).
650    _2
$a techniky typizace bakterií $7 D015373
650    _2
$a zastoupení bazí $7 D001482
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a ekosystém $7 D017753
650    _2
$a mastné kyseliny $x chemie $7 D005227
650    12
$a Pedobacter $7 D060485
650    _2
$a fylogeneze $7 D010802
650    _2
$a RNA ribozomální 16S $x genetika $7 D012336
650    _2
$a sekvenční analýza DNA $7 D017422
651    _2
$a Antarktida $7 D000864
655    _2
$a časopisecké články $7 D016428
700    1_
$a Králová, Stanislava $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Staňková, Eva $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Holochová, Pavla $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Sedlář, Karel $u Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 12, 616 00 Brno, Czech Republic
700    1_
$a Koudelková, Sylva $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Krsek, Daniel $u NRL for Diagnostic Electron Microscopy of Infectious Agents, The National Institute of Public Health, Šrobárova 49/48 100 00 Prague 10, Czech Republic
700    1_
$a Grzesiak, Jakub $u Department of Antarctic Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland
700    1_
$a Šedo, Ondrej $u Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Váczi, Peter $u Department of Experimental Biology, Division of Experimental Plant Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
700    1_
$a Urvashi, $u Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India
700    1_
$a Gupta, Vipin $u Ministry of Environment Forest & Climate Change, Integrated Regional Office, Dehradun, 248001, India
700    1_
$a Sood, Utkarsh $u The Energy and Resources Institute, Lodhi Road New Delhi-110003, India
700    1_
$a Lal, Rup $u The Energy and Resources Institute, Lodhi Road New Delhi-110003, India
700    1_
$a Korpole, Suresh $u Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India
700    1_
$a Sedláček, Ivo $u Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
773    0_
$w MED00006002 $t International journal of systematic and evolutionary microbiology $x 1466-5034 $g Roč. 72, č. 4 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35442878 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804135228 $b ABA008
999    __
$a ok $b bmc $g 1822538 $s 1170224
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 72 $c 4 $e - $i 1466-5034 $m International journal of systematic and evolutionary microbiology $n Int. j. syst. evol. microbiol. (Print) $x MED00006002
LZP    __
$a Pubmed-20220720

Find record

Citation metrics

Archiving options