-
Something wrong with this record ?
Increased macrophage M2/M1 ratio is associated with intracranial aneurysm rupture
MH. Stratilová, M. Koblížek, A. Štekláčová, V. Beneš, M. Sameš, A. Hejčl, J. Zámečník
Language English Country Austria
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Medline Complete (EBSCOhost)
from 2000-01-01
Springer Nature OA/Free Journals
from 1950-02-01
- MeSH
- Intracranial Aneurysm * complications pathology MeSH
- Humans MeSH
- Macrophages * pathology MeSH
- Aneurysm, Ruptured * complications pathology MeSH
- Thrombosis complications MeSH
- Inflammation complications MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
PURPOSE: Intracranial aneurysm (IA) rupture results in one of the most severe forms of stroke, with severe neurological sequelae. Inflammation appears to drive aneurysm formation and progression with macrophages playing a key role in this process. However, less is known about their involvement in aneurysm rupture. This study is aimed at demonstrating how relationship between the M1 (pro-inflammatory) and M2 (reparative) macrophage subtypes affect an aneurysm's structure resulting in its rupture. METHODS: Forty-one saccular aneurysm wall samples were collected during surgery including 13 ruptured and 28 unruptured aneurysm sacs. Structural changes were evaluated using histological staining. Macrophages in the aneurysm wall were quantified and defined as M1 and M2 using HLA-DR and CD163 antibodies. Aneurysm samples were divided into four groups according to the structural changes and the M2/1 ratio. Data were analyzed using the Mann-Whitney U test. RESULTS: This study has demonstrated an association between the severity of structural changes of an aneurysm with inflammatory cell infiltration within its wall and subsequent aneurysm rupture. More severe morphological changes and a significantly higher number of inflammatory cells were observed in ruptured IAs (p < 0.001). There was a prevalence of M2 macrophage subtypes within the wall of ruptured aneurysms (p < 0.001). A subgroup of unruptured IAs with morphological and inflammatory changes similar to ruptured IAs was observed. The common feature of this subgroup was the presence of an intraluminal thrombus. CONCLUSIONS: The degree of inflammatory cell infiltration associated with a shift in macrophage phenotype towards M2 macrophages could play an important role in structural changes of the aneurysm wall leading to its rupture.
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
International Clinical Research Center St Anne's Hospital Brno Czech Republic
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23004748
- 003
- CZ-PrNML
- 005
- 20240703104209.0
- 007
- ta
- 008
- 230418s2023 au f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s00701-022-05418-0 $2 doi
- 035 __
- $a (PubMed)36437400
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a au
- 100 1_
- $a Hundža Stratilová, Mária, $u Department of Neurosurgery, J. E. Purkyne University, Masaryk Hospital, Sociální péče 3316/12A, 400 13, Ústí Nad Labem, Czech Republic $u Department of Pathology and Molecular Medicine, 2nd Faculty of Medicine, Charles University, and Motol University Hospital, Prague, Czech Republic $d 1990- $7 xx0228529
- 245 10
- $a Increased macrophage M2/M1 ratio is associated with intracranial aneurysm rupture / $c MH. Stratilová, M. Koblížek, A. Štekláčová, V. Beneš, M. Sameš, A. Hejčl, J. Zámečník
- 520 9_
- $a PURPOSE: Intracranial aneurysm (IA) rupture results in one of the most severe forms of stroke, with severe neurological sequelae. Inflammation appears to drive aneurysm formation and progression with macrophages playing a key role in this process. However, less is known about their involvement in aneurysm rupture. This study is aimed at demonstrating how relationship between the M1 (pro-inflammatory) and M2 (reparative) macrophage subtypes affect an aneurysm's structure resulting in its rupture. METHODS: Forty-one saccular aneurysm wall samples were collected during surgery including 13 ruptured and 28 unruptured aneurysm sacs. Structural changes were evaluated using histological staining. Macrophages in the aneurysm wall were quantified and defined as M1 and M2 using HLA-DR and CD163 antibodies. Aneurysm samples were divided into four groups according to the structural changes and the M2/1 ratio. Data were analyzed using the Mann-Whitney U test. RESULTS: This study has demonstrated an association between the severity of structural changes of an aneurysm with inflammatory cell infiltration within its wall and subsequent aneurysm rupture. More severe morphological changes and a significantly higher number of inflammatory cells were observed in ruptured IAs (p < 0.001). There was a prevalence of M2 macrophage subtypes within the wall of ruptured aneurysms (p < 0.001). A subgroup of unruptured IAs with morphological and inflammatory changes similar to ruptured IAs was observed. The common feature of this subgroup was the presence of an intraluminal thrombus. CONCLUSIONS: The degree of inflammatory cell infiltration associated with a shift in macrophage phenotype towards M2 macrophages could play an important role in structural changes of the aneurysm wall leading to its rupture.
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a prasklé aneurysma $x komplikace $x patologie $7 D017542
- 650 _2
- $a zánět $x komplikace $7 D007249
- 650 12
- $a intrakraniální aneurysma $x komplikace $x patologie $7 D002532
- 650 12
- $a makrofágy $x patologie $7 D008264
- 650 _2
- $a trombóza $x komplikace $7 D013927
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Koblížek, Miroslav $u Department of Pathology and Molecular Medicine, 2nd Faculty of Medicine, Charles University, and Motol University Hospital, Prague, Czech Republic
- 700 1_
- $a Štekláčová, Anna $u Department of Neurosurgery and Neurooncology, Military University Hospital and Charles University, First Medical Faculty, Prague, Czech Republic
- 700 1_
- $a Beneš, Vladimír $u Department of Neurosurgery and Neurooncology, Military University Hospital and Charles University, First Medical Faculty, Prague, Czech Republic
- 700 1_
- $a Sameš, Martin $u Department of Neurosurgery, J. E. Purkyne University, Masaryk Hospital, Sociální péče 3316/12A, 400 13, Ústí Nad Labem, Czech Republic
- 700 1_
- $a Hejčl, Aleš $u Department of Neurosurgery, J. E. Purkyne University, Masaryk Hospital, Sociální péče 3316/12A, 400 13, Ústí Nad Labem, Czech Republic. ales.hejcl@gmail.com $u International Clinical Research Center, St. Anne's Hospital, Brno, Czech Republic. ales.hejcl@gmail.com $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. ales.hejcl@gmail.com $1 https://orcid.org/0000000292061980 $7 xx0077402
- 700 1_
- $a Zámečník, Josef $u Department of Pathology and Molecular Medicine, 2nd Faculty of Medicine, Charles University, and Motol University Hospital, Prague, Czech Republic
- 773 0_
- $w MED00009022 $t Acta neurochirurgica $x 0942-0940 $g Roč. 165, č. 1 (2023), s. 177-186
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36437400 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230418 $b ABA008
- 991 __
- $a 20240703104204 $b ABA008
- 999 __
- $a ok $b bmc $g 1925066 $s 1190957
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 165 $c 1 $d 177-186 $e 20221128 $i 0942-0940 $m Acta neurochirurgica $n Acta Neurochir $x MED00009022
- LZP __
- $a Pubmed-20230418
